Photogrammetry, a technique to obtain measurements from photographs, may be a valid method for measuring lengths of rare, threatened, or endangered species. Photogrammetric methods of measurement are nonintrusive and reduce the possibility of physical damage or physiological stress associated with the capture and handling of individuals. We evaluated precision and accuracy of photogrammetric length measurements relative to board measurements of Greenside Darters Etheostoma blennioides and Variegate Darters E. variatum in an aquarium and applied photogrammetry in a field study of the Diamond Darter Crystallaria cincotta, a federally listed endangered species. Digital photographs were taken of each individual using a waterproof camera equipped with two parallel lasers. Photogrammetric length measurements were digitized with ImageJ software. Agreement between board and photogrammetric measurements were high for Greenside and Variegate darters. The magnitude of differences was small between direct and photogrammetric measurements, ranging from 0.6% to 3.1%, depending on the species measured and the type of measurement taken. These results support photogrammetry as a useful method for obtaining length measurements of benthic stream fishes. Photogrammetric methods allowed for length measurements and an assessment of length frequency of 199 Diamond Darters, informative data for management that could not be collected with conventional measuring-board methods.

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The western sand darter, Ammocrypta clara, and the eastern sand darter, A. pellucida, are sand-dwelling fishes that have undergone range-wide population declines, presumably owing to habitat loss. Habitat use studies have been conducted for the eastern sand darter, but literature on the western sand darter remains sparse. To evaluate substrate selection and preference, western and eastern sand darters were collected from the Elk River, West Virginia, one of the few remaining rivers where both species occur sympatrically. In the laboratory, individuals were given the choice to bury into five equally available and randomly positioned substrates ranging from fine sand to granule gravel (0.12-4.0 mm). The western sand darter selected for coarse and medium sand, while the eastern sand darter was more of a generalist selecting for fine, medium, and coarse sand. Substrate selection was significantly different (p = 0.02) between species in the same environment, where the western sand darter preferred coarser substrate more often compared to the eastern sand darter. Habitat degradation is often a limiting factor for many species of rare freshwater fish, and results from this study suggest that western and eastern sand darters may respond differently to variations in benthic substrate composition.

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