Accumulation of body fat is the major problem associated with initiation of various diseases in fast growing broiler chickens. Methods to restrict the accumulation of body fat have met with limited success. The objective of this study was to evaluate the effect of Avocado Non-Saponifiable Fraction (NSF) in broiler chicken (from day 14 for 27 days) on body fat accumulation, growth performance, organ weight and plasma lipid profile. Supplementation of avocado NSF for 27 days has resulted in positive changes in plasma lipid profile with 50% decrease of abdominal fat and 33.8% increase in body weight compared with normal control chickens. The Avocado NSF improved body composition in broilers through higher lean-to-fat ratio and hence has a potential to improve overall health profile and reduce metabolic diseases in chickens.