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Plant based phytoconstituents of great importance in the development of drugs and treatment of several health diseases.In this study, the phytochemical constituents of ethanol extract of Celosia leptostachya leaves, invitro antioxidant and alpha amylase inhibitory properties were investigated. Gas chromatography-mass spectrum analysis was used to identify varying percentage composition of phytochemical constituents in the extract. In vitro models were used to evaluate the ability of varying concentrations of the ethanol extracts to exhibit antioxidant activity by free radical scavenging using nitric oxide (NO) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Major constituents are cis-3-Butyl-4-vinyl-cyclo pentene n-Hexadecanoic acid, 9,12-Octadecadienoic acid (Z,Z) , Bicyclo[2.2.1]heptane, 7,7-dimethyl-2-methylene, octadecanoic acid (7.47%), arachidonic acid and 9-octadecenoic acid (E) (5.40%). Many of the constituents are also representative compounds belonging to fatty acid esters and volatile oils. Invitro antioxidant studies using DPPH and NO radical scavenging activity indicated that the extract exhibited > 50% inhibition at concentrations of 500 µg/ml and 600 µg/ml. IC50 values obtained for the extracts indicated strong antioxidant activity. Alpha amylase inhibition showed that increase in concentration of the extract exhibited a corresponding increase in percentage inhibition of the enzyme similar to acarbose used as standard. The kinetic studies under inhibitory conditions showed a decrease in Km and Vmax from slope and noncompetitive. Thus, it can be deduced that ethanol extract of Celosia leptostachya leaves contained essential phytochemical components that can be explored for broad spectrum bioactivity, pharmacological and nutritional applications. The inhibition mechanism is noncompetitive and can be utilized as strategy in control and treatment of hyperglycemia.

Background: Sodium nitroprusside (SNP) is an antihypertensive agent. It induces toxicity via the release of cyanide ions, nitric oxide (NO) and reactive oxygen species. Manniophyton fulvum (MF) is commonly used in Nigeria due to its therapeutic and nutritional potentials. This study evaluates the phytochemical composition of aqueous extract of MF root and influence in SNP induced oxidative stress in wistar rats.Methods: Gas chromatography was used for determination of the chemical composition of aqueous extract of MF root. Rat liver homogenate was used for determination of rhodanese, glutathione (GSH) and malondialdehyde (MDA). Twenty (20) rats were randomly divided into four different groups comprising five animals (n=5). Control (Group A), Groups B, C, D received 2.5 mg/kg body weight of SNP at intervals of three hours per day by intraperitoneal injection. In addition, Groups C and D received 200 mg/kg body weight of aqueous root extract of MF and 10 mg/kg body weight of Vitamin E respectively for a period of seven days.Results: Flavonoids had the highest composition while allicin had the lowest composition. Diallyl thiosulphinate>methyl allyl thiosulphinate >allyl methyl thiosulphinate. The activity of rhodanese, GSH and MDA concentrations showed that Group B had significant (p<0.05) increase in MDA concentration while GSH showed significant (p<0.05) decrease. Also, the activity of rhodanese showed significant (p<0.05) decrease compared to Group A. However, Groups C and D showed significant increase (p<0.05) in the activity of rhodanese enzyme compared to Group A and Group B. GSH levels of Group C and Group D showed no significant (p>0.05) difference while the MDA concentration showed significant (p<0.05) decrease. Correlation analysis between rhodanese and GSH showed strong significant (p=0.01, r=0.894) positive correlation.Conclusions: It can be deduced that the chemical components of aqueous extract of MF may serve as a pharmacological agent for molecular targets to up regulate detoxification and cytoprotective enzymes. Cellular localization of rhodanese enzyme can be induced by aqueous extract of MF root to collaborate with GSH and promote inhibition of lipid peroxidation, anti-oxidative reactions and up regulate cyanide detoxification in tissues.

This study explored the protective potential of NADPH-oxidase inhibitors, apocynin and curcumin in diclofenac-induced cardiotoxicity via oxidative stress. A total of 80 male Wistar rats were used for the study. 80 rats were randomly divided into 8 groups of 10 rats each. Group 1(control) received distilled water while others received orally, per mg/kg body weight of treatments as follows: group 2(1000, apocynin, group 3(1000, curcumin), group 4(10, diclofenac), group 5(500, apocynin and 10, diclofenac), group 6(1000, apocynin and 10, diclofenac), group 7(500, curcumin and 10, diclofenac) and group 8(1000, curcumin and 10, diclofenac). The treatments were administered daily for 14 and 28 days. Administration of diclofenac significantly (p<0.05) elevated the activities of NAD(P)H oxidases type 2 and malondialdehyde while the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione level were significantly (p<0.05) decreased. There was no alteration in the activities of xanthine oxidase. However, pretreatment with 500 and 1000 mg/kg body weight of apocynin or curcumin attenuated all biochemical alterations induced by diclofenac in a dose dependent manner. Pretreatments with apocynin and curcumin inhibitors of NOX 2 was effective in ameliorating diclofenac-induced cardiotoxicity by alleviating the oxidative stress thus, highlighting the therapeutic potentials of apocynin and curcumin in the management of diclofenac-mediated cardiotoxicity.