We have designed and fabricated a library of polyethylene glycol (PEG)-based polymer blends, including blends of two PEG-based polymers that are liquid at room temperature where the optimisation of the blending method allows for the incorporation of higher molecular-weight PEG-based polymers which are solid at room temperature. The absence of a solvent in these blends makes them perfect candidates for use in our recently developed Fill-Molding in Capillaries (FIMIC) patterning method. As our FIMIC samples have shown to be not completely smooth (a small topography up to several nanometers has been seen previously), and this is likely to affect the cellular behaviour, we have improved our technique in order to obtain virtually smooth samples that exhibit a pattern of elasticity only. It is demonstrated that, by taking advantage of the differential swelling of the pattern components, we can level out the undesired topographic difference. In particular, by employing blends of materials, (1) the swelling degree of each component can be fine-tuned to even out any topography and (2) the use of the same blends in the sample, yet with varying cross-linker amounts, ensures the swelling degree and elasticity change without changing the surface chemistry significantly. Genuine, binary patterns of elasticity can thus be fabricated, which are a great asset to study cell migration phenomena in systematic detail.

In this study, a mask-less laser-assisted patterning method is used to fabricate welldefined cell-adhesive microdomains delimited by protein-repellent poly(ethylene glycol) (PEG) microstructures prepared from multiarm (8-PEG) macromonomers. The response of murine fibroblasts (L-929) toward these microdomains is investigated, revealing effective cell confinement within the celladhesive areas surrounded by nonadhesive 8-PEG microstructures. Moreover, the spatial positioning of cells in microdomains of various sizes and geometries is analyzed, indicating control of cell density, size, and elongated cell shape induced by the size of the microdomains and the geometric confinement.

No abstract

No abstract