We investigated performance levels on conducting continuous two-footed jumping of preschool children (4 years old) to high school students (16 years old) to clarify the developmental progression and sex differences in motor coordination and agility. In total, 450 children (boys: 227; girls: 223) participated in this study. We set 10 obstacles to jump over for continuous two-footed jumping and analyzed the movement time (MT), aerial time (AT), and contact time (CT), and variabilities in AT and CT in 7 year-based categories, using a high-speed camera. We also used multiple regression analysis to identify the predictors of MT. MT and CT shortened until 8 years, whereas AT continued to shorten after 8 years, suggesting that the jumping strategy differs between those younger/older than 8 years. MT, AT, and CT were significantly shorter among boys than girls from preschool children to high school students. In addition, when using multiple regression analysis, the main predictor of MT changed gradually from SD of CT to AT with increasing age. Our findings suggest that the motor control mechanisms related to continuous two-footed jumping differ depending on the age and sex and provide findings to advance understanding of the age-related motor coordination and agility in children.