Silver nanoparticles were synthesized using green tea leaf extract and were characterized using different analytical techniques. The synthesized nanoparticles showed antibacterial activity against Staphylococcus aureus and Klebsiella spp.
The aim of this study was to find out the nutritional value, fatty acids and mineral composition of fruits of Capparis decidua L. This has been carried out by analyzing chemical composition for samples of the plant collected from Goz Abu kelab, Algutaina Road, White Nile State, Sudan. The proximate analysis and fatty acids composition were determined by analyzing sample of identified plants using different methods. The results of analysis showed that the fruits of C. decidua. contain total ash (6.34±0.06%), moisture (5.18±0.01%), total oil percentage (6.02±0.02%), crude fiber (6.19±0.5%), crude protein (14.32±0.2%), total carbohydrates (61.95±0.03%) and total sugar (0.42±0.1%). The fixed oil extracted from fruits were evaluated for chemical composition. The GC-FID analysis showed the presence of various saturated and unsaturated fatty acids such as Lauric acid (0.2080%) methyl ester, Tri decanoic acid methyl ester (0.3035%), Myristic acid methyl ester (0.5851%), palmatic acid methyl ester (46.0220%), Lamda-Linolenic acid methyl ester (0.6700%), Linolelaidic acid methyl ester (45.7910%), Cis-11-Eicosenoic acid methyl ester (3.9941%), Arachidonic acid methyl ester (0.3080%), Erucic acid methyl ester (1.6876%) and Tricosanoic acid methyl ester (0.4307%). Fruits of C.decidua contained iron (81.8 mg/100g), sodium (26.9 mg/100g), potassium (2969.6 mg/100g), calcium (14.1 mg/100g) and manganese was not detected but it might been found in trace undetectable amounts. Generally, proximate compositions revealed that C.decidua fruits have better nutritional value as food and livestock feedstuff.
Synthesis of silver nanoparticles using seeds of Nigella sativa as a capping agent was evaluated in this study. Different concentrations of the aqueous extract of N. sativa with silver nitrate solution were exposed to sunlight; as a force for acceleration of the formulation. Then the silver nanoparticles were characterized by UV-Vis, scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. Antibacterial activity of the nanoparticles was investigated against Staphylococcus aureus and Escherichia coli by the disc diffusion method. The characterization of nanoparticles was detected by the change in color to yellow-brown which indicated the formulation of silver nanoparticles. Irregular shapes within range of nanoscale were detected using SEM and XRD techniques. The finding suggests that silver nanoparticles may be effectively used as antibacterial agent.
Punica granatum L. (Pomegranate) is a plant belongs to Lythraceae family. The objective of this study was to evaluate the antimicrobial activity of petroleum ether, chloroform , acetone, ethyl acetate and ethanol extracts from the peel fruit of Punica granatum against standard microorganism. This plant has been used as a traditional treatment for several diseases such as microbial infections. Extracts were evaluated for their effectiveness against four bacterial strains including both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria as well as fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. The ethyl acetate, acetone and petroleum ether extracts showed higher antibacterial activity than the chloroform and ethanol extracts with inhibition zone ranged between (7-16 mm). The petroleum ether and chloroform extracts showed the higher antifungal activity compared with the other extracts, the diameter inhibition zone ranged between (11-16 mm). The antioxidant potential of peel extracts was determined on the basis of their scavenging activity of the stable 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical and 2,2'azino-bis(ethyl benzthiazoline -6-sulfonic acid (ABTS+). All extracts showed high antioxidant activity . The highest result of antioxidant activity by DPPH scavenging assay was in acetone extract (89.40%), but high result by ABTS in ethanol extract (72.07%).
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