P akistan has a lot of different plants and animals because it has a lot of good land for farming. A lot of crops and species aren't used for business or money. Cannabis sativa is one of those crops in Pakistan that isn't very well-known with the name of Bhang, even though it has a lot of good things about it. There is Abstract | Cannabis sativa is a high-value crop that grows naturally in Pakistan's highlands. This is commonly known "Bhang" in Pakistan. This study is an attempt to recognize the C. sativa plant's wonderful physiochemical qualities and socioeconomic benefits. Antioxidants abound in C. sativa extract, thanks to its high phenolic content. It's a high-polyunsaturated acid crop that's strong in antioxidants. Extraction from C. sativa seeds yielded a 33% yield of C. sativa oil, which was then analyzed for TPC and antioxidants in comparison to commercially available C. sativa oil. The Folin-Ciocalteu reagent and the α, α-diphenyl-β-picrylhydrazyl (DPPH) test were used to measure the total phenolic content of oil and leaf extracts. Bioactive components included in plant extracts, such as tocopherol, chlorophyll, carotenoids, phenolics, flavonoids, and other scavenging components, make them more potent. Thus, the 50µg/ml concentration plant extract solution has the lowest radical scavenging percentage, whereas the 1000µg/ml concentration plant extract solution has the highest DPPH percentage 80%. The results of the test reveal that the extract concentration had an influence on the assay's absorbance, or, to put it another way, an increase in extract concentration produces a drop in absorbance, demonstrating an inverse relationship. The extract's antioxidants inhibit DPPH, causing the absorbance to gradually diminish. Our study described that C. sativa have a wide range of volatile compounds with multiple range of physiochemical qualities and socioeconomic benefits so our study emphasizes on the future work with belief that C. sativa is a valuable crop that should be further investigated for commercial and culinary purposes.

Protein Kinase C (PKC) and tubulin homologs are present in all eukaryotes and play significant role in growth, development and cell differentiation through phosphorylation and de-phosphorylation of other proteins. In this study, we have amplified PKC and beta tubulin homolog bml gene from six strains and F3 and F4 generations of Sordaria fimicola collected from the Evolution Canyon-1. Sequenced products of 464 bp of tubulin gene and 548 bp for PKC gene were aligned to observe the genetic variations between the eight parental strains of S. fimicola and reference sequence of S. fimicola. Total six polymorphic sites were observed in case of tubulin gene out of which 5 sites were common among strains isolated from the s-slope of Evolution Canyon (EC). Genetic variations in four nucleotides were observed for PKC gene i.e. C (150) T; C (186) A; C (429) G and T (521) A which were common for S1, S2 and S3 strains, while point mutation C (497) G was detected only in S2 and S3 strains. Post translational modifications (PTMs) of both proteins were predicted and compared with the reference sequences of Neurospora crassa and Sordaria macrospora by using different PTMs predictor servers. Phosphorylation and glycosylation in different species of Sordaria as well as N. crassa was calculated on Serine (S), Tyrosine (Y) and Threonine (T) residues by NetPhos and YinOYang.