Recent evidence suggests early environmental factors are important for gut immune tolerance. Although the role of regulatory T (Treg) cells for gut immune homeostasis is well established, the development and tissue homing characteristics of Treg cells in children have not been studied in detail. In this article, we studied the development and homing characteristics of human peripheral blood Treg cell subsets and potential mechanisms inducing homing molecule expression in healthy children. We found contrasting patterns of circulating Treg cell gut and skin tropism, with abundant β7 integrin Treg cells at birth and increasing cutaneous lymphocyte Ag (CLA) Treg cells later in life. β7 integrin Treg cells were predominantly naive, suggesting acquisition of Treg cell gut tropism early in development. In vitro, IL-7 enhanced gut homing but reduced skin homing molecule expression in conventional T cells, whereas IL-2 induced a similar effect only in Treg cells. This effect was more pronounced in cord compared with adult blood. Our results suggest that early in life, naive Treg cells may be driven for gut tropism by their increased sensitivity to IL-2-induced β7 integrin upregulation, implicating a potential role of IL-2 in gut immune tolerance during this critical period of development.

The study aims to recognize bacterial colonization of surfaces and equipment’s in operation theaters and to determine the bacteriological contamination of air in OTs in Gojra. Objectives: To identify the bacteriological colonization of surfaces, inanimate objects, air, in the operation room facilities and antibiotic susceptibility profile of the isolates at tehsil Gojra hospitals. Study Design: One Group Pre and Post-experimental study. Settings: Department of Surgery THQ Hospital Gojra. Period: 1st January 2019 to 31st July 2019. Material & Methods: Operation theatres sampled and left vacant for more than one-hour before sampling. Settle pate method used for sampling of air by using agar plates and swab method for sampling of surfaces and equipment. Bacterial isolates identified by colony morphology. Gram Staining, Antibiotic Susceptibility testing done by disc diffusion technique. Conclusion: There is a high hazard for the occurrence of surgical site infections because of the significant level of pollution observed in our study. The results emphasize the need for proper cleaning, scrubbing techniques, improved disinfection and sterilization methods and regular fumigation of operation theatres to prevent the cause of infection and maintain a safe environment for the patients and health care professionals.

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