Bacillus subtilis is a rod shaped, gram positive, spore producing bacterium. They are the normal flora of gastrointestinal tract of humans and it is the best characterized model organism for endospore formation. It has the ability to withstand environmental stress, and synthesize beneficial compounds, therefore, it is recognized as a high-quality probiotic supplement. To ensure the probiotic safety and the efficiency, we report the whole genome sequence (WGS) of Bacillus subtilis UBBS-14 strain. The draft genome sequence of Bacillus subtilis UBBS-14 consists of 4,048,984 bp and 4,017 genes, respectively. Bacillus subtilis UBBS-14 does not carry any antibiotic resistant genes containing plasmid, nor it contains any harmful putative virulence factors coding genes, therefore, it confirms the probiotic safety of the respective strain at genome level.

Wastewater treatment plant monitoring is an essential part of effective wastewater management. The analysis of eight physico-chemical parameters of untreated wastewater was carried out at Vidyaranyapuram sewage treatment plant, Mysore, India. Factor analysis (FA) was applied to the untreated wastewater data matrix, and pollution was found to be the most contributing factor, explaining 22.31% of the total variance (chloride, biochemical oxygen demand, chemical oxygen demand and total dissolved solids). The second most contributing factor was found to be nitrification which explained 21.11% of the total variance (pH and nitrate), whereas the salinization factor contributed 16.98% of the total variance (total solids and total suspended solids). FA regression scores could not satisfactorily classify the data matrix with respect to the seasonal variations. Discriminant analysis (DA) was used to find the seasonal variations in the data matrix, and the standard mode DA explained 66.6% of total variance by grouping the cases with respect to seasons.

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Clostridium butyricum is a strictly anaerobic, butyric acid-producing, Gram-positive, spore-forming bacillus that is commonly present in the gut of humans. The complete genome sequence of Clostridium butyricum UBCB 70 was studied to evaluate the presence of antibiotic-resistant and clostridium toxin genes.