The objective of this study was to identify seminal plasma proteins associated with semen traits (mass motility (MM; %), live sperm (LS; %), abnormal sperm (AS; %), hypo-osmotic swelling test (Host; %), pH, colour, viscosity (Visco), ejaculate volume (VOL; ml), normal chromatin (NC; %), live sperm with reacted acrosome (LR; %), live sperm with unreacted acrosome (LU; %), dead sperm with reacted acrosome (DR; %), dead sperm with unreacted acrosome (DU; %)) in Egyptian Barki rams. Depending on MM%, the animals were divided into three different groups (high (≥ 80% MM), medium (50-80% MM) and low-fertility (≤50% MM) group). The seminal plasma proteins were separated using one-dimensional sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis technique based on their molecular weight. The actual means