Background and Objective: Psychoactive substances are chemical substances that change brain function and result in alterations in perception, mood, consciousness, cognition or behaviour when taken. This study evaluated the effects of psychoactive substances in the white tips of lizard dung, Indian hemp, Datura metel seed and blue dye on some biochemical parameters and histology of male albino rats. Materials and Methods: Twenty-five male albino rats weighing 91±9.4 g were used for the study and allotted into five groups with group 1 as control which received potable water, while groups 2-5 received potable water, a mixture of lizard dung with other substances. The substances were administered to the experimental animals for14 days, at the end of which they were sacrificed, serum samples were collected for biochemical analysis, while the kidney was collected for histological study. Results: There were no significant differences in serum electrolyte levels of calcium, sodium, potassium and chloride for control when compared with other experimental groups. The results of the malondialdehyde showed that there was a significant difference (p<0.05) between the control and groups 3 and group 4 and no significant difference (p>0.05) between the control and groups 2 and 5. Conclusion: The study revealed that varying types of psychoactive substances elicited varying concentrations of malondialdehyde which reflected the levels of peroxidation. However, this variation among treatments was not significantly different (p>0.05). The study further revealed no aberration in the histology of the kidney suggesting that psychoactive substances have no negative impact in albino rats fed for 14 days.