The aim of this study was to evaluate relations between color distributions in the cervical, middle, and incisal segments of the teeth in the anterior region. Material and Method: Thirty-one individuals aged between 18-24 participated in the study. Color measurements were performed using VITA Easyshade V (VITA Zahnfabrik, Germany) from the cervical, middle and incisal regions of the left upper central, lateral, and canine teeth of participants. L*, a*, b* values were determined using a spectrophotometer. ΔE values were calculated to determine the color difference, and L*, a*, b * values were statistically analyzed. Results: Statistically significant results were obtained between L*, a*, b* values of different teeth segments using Spearman's correlation analysis. ΔE was calculated higher than 3.7 among all teeth segments. Conclusions: Using the L* and b* values of the middle segment color of the upper lateral tooth, it is possible to calculate the L* and b* values of the incisal, middle, and cervical colors of the upper incisors and canines.