No abstract

Two Gram-stain-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria designated PDF4T and PDF10, were isolated from Camkoy hot spring in the provinces of Aydın, Turkey and were characterized in order to determine their phylogenetic position. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus. Strain PDF4 T showed highest 16S rRNA gene sequence similarity to strain PDF10 (99.5 %),Brevibacillus brevis DSM 30 T (98.9 %), Brevibacillus parabrevis DSM 8376 T (98.6 %) andBrevibacillus formosus DSM 9885 T (98.5 %); similarities to other species of the genus Brevibacillus were less than 98.5 %. The predominant fatty acids of strain PDF4 T were anteiso-C 15 : 0 (60.0 %) and iso-C 15 : 0 (22.3 %). The polar lipids of strain PDF4 T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unknown phospholipid, two unknown lipid, an unknown aminophospholipid and two unknown aminolipids. MK-7 was detected as a sole respiratory quinone, and the cell wall of strain PDF4 T contained meso-diaminopimelic acid. The DNA G+C content of strain PDF4 T was 51.7 mol%. DNA-DNA hybridization showed less than 60 % relatedness between strain PDF4 T and type strains of the most closely related species given above. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus, for which the name Brevibacillus gelatini sp. nov. is proposed. The type strain is PDF4 T (5NCCB 100559 T 5DSM 100115 T ).

No abstract

No abstract

A Gram-staining-negative, non-motile, catalase-and oxidase-positive strain, designated MS7 T , was isolated from freshwater of a river near Trabzon, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew optimally at 28 8C and pH 7.5 and in the presence of 2.0 % NaCl. 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Algoriphagus; strain MS7T showed highest sequence similarity to the type strains of Algoriphagus alkaliphilus (97.3 %), Algoriphagus terrigena (96.8 %), Algoriphagus jejuensis (96.2 %), Algoriphagus boritolerans (96.1 %) and Algoriphagus aquatilis (95.8 %). The major fatty acids of strain MS7 T were iso-C 15 : 0 (30.14 %) and summed future 9 (10-methyl C 16 : 0 and/or iso-C 17 : 1 v9c 18.75 %). Polar lipid analysis revealed phosphatidylethanolamine, a variety of unidentified lipids, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified aminolipid. The major isoprenoid quinone was MK-7.The DNA G+C content of MS7 T was 41.6 mol%, a value consistent with that of members of the genus Algoriphagus. The level of DNA-DNA relatedness between strain MS7 T and A. alkaliphilus LMG 22694 T was 41 %, which is clearly below the 70 % threshold accepted for species delineation. Thus, our results support the placement of strain MS7 T within a separate and previously unrecognized species. On the basis of these data, the strain is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus trabzonensis sp. nov. is proposed. The type strain is MS7 T (5NCCB 100372 T 5LMG 26290 T ). An emended description of A. alkaliphilus is also provided.