AbstrakKelurahan Lubuk Buaya merupakan daerah endemik Demam Berdarah Dengue (DBD) dengan korban meninggal terbanyak pada tahun 2012. Penyebaran DBD dipengaruhi oleh faktor lingkungan dan tindakan Pemberantasan Sarang Nyamuk (PSN). Tujuan penelitian ini adalah menentukan hubungan tindakan pemberantasan sarang nyamuk dan keberadaan larva vektor DBD di Kelurahan Lubuk Buaya Kecamatan Koto Tangah Kota Padang. Jenis penelitian adalah analitik observasional dengan rancangan cross sectional. Penelitian dilaksanakan di Kelurahan Lubuk Buaya dengan 110 sampel pada bulan Desember 2014. Sampel diambil dengan metode Multistage Random Sampling. Pengambilan data menggunakan kuesioner dan survei larva terhadap kontainer yang berada di dalam dan di luar rumah responden. Data disajikan dalam bentuk tabel ditribusi dan dianalisis statistik dengan uji chi-square. Hasil penelitian menunjukkan lebih dari separuh responden melakukan tindakan PSN yang baik. Keberadaan larva vektor DBD tergolong tinggi dengan HI 35,45%, CI 13,41%, BI 50% dan Density figure/Df= 5. Terdapat hubungan yang bermakna antara tindakan pemberantasan sarang nyamuk dengan keberadaan larva vektor DBD di kelurahan Lubuk Buaya (p=0,001). Pelaksanaan PSN di Kelurahan Lubuk Buaya secara umum belum terlaksana secara optimal.Kata kunci: PSN, larva, vektor DBD AbstractKelurahan Lubuk Buaya is a Dengue Hemorhagic Fever (DHF) endemic area with the highest death case in 2012. The spreading of DHF influenced by environmental factor and practice of mosquito breading place eradication. The objective of this study was to determine the relationship between mosquito breading place eradication practice and the presence of larvae DHF’s vector. The research was an analitic observational with cross-sectional study design. The research was held in Lubuk Buaya with 110 samples in December 2014. The samples were taken with the Multistage Random Sampling methods. Data’s were collected by using a questionnare and survey of the larvae. Data were presented in distribution table and analyzed statistically with Chi Square method. The result showed more than half of the respondents have a good mosquito breading place eradication practice and the presence of larvae DHF’s vector in lubuk buaya is high with HI 35.45%, CI 13.41%, BI 50%, and density figure 5. There is a relationship between breading place eradication practice and the presence of larvae DHF’s vector (p=0.001). The implementation of breading place eradication practice in Lubuk Buaya isn’t implemented optimally.Keywords: breading place eradication, larvae, dengue hemorhagic fever vektor