No abstract

Disuse condition leads to decrease fiber cross‐sectional area and satellite cell activity in skeletal muscle. Transcutaneous electrical stimulation (ES) can suppress unloading‐induced muscle atrophy, but not prevent. Lemon myrtle (LM), one of herbs, could activate muscle satellite cells in our previous study. Therefore, the preventive effects of the lemon myrtle supplementation on muscle atrophy were investigated. In addition, we determined the activation in muscle satellite cells, the number of myonuclei, and the prevention of disuse‐induced muscle atrophy by the combination of LM supplementation and ES. Wistar rats were divided into 5 groups; control (CON), hindlimb unloading (HU), HU + LM, HU + ES and HU + LM + ES groups. The experiment period was 14 days. Unloading condition resulted in a decrease in the fiber cross‐sectional area (FCSA). The FCSA was significantly increased in HU + LM + ES group than in the other three groups (HU, HU + LM and HU + ES). In addition, the expression levels of Pax 7, MyoD and myogenin were higher in HU + LM + ES group compared with the other three groups (HU, HU + LM and HU + ES). Furthermore, the number of myonuclei were significantly decreased in HU, HU + LM and HU + ES groups than in CON group although there was no significant difference between in HU + LM + ES group and CON group. These results suggest that the combination of lemon myrtle supplementation and electrical stimulation suppressed the reduction of the fiber cross‐sectional area compared with lemon myrtle supplementation or electrical stimulation alone, and these alterations were accompanied with an increase in the number of myonuclei associated with the activation of muscle satellite cells.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Abstract:The applications of immunoglobulin preparation for intravenous injection (IVIg) for various intractable diseases are increasing. The two major clinical indications for IVIg are the replacement therapy and the anti-inflammation therapy for a variety of acute and chronic autoimmune diseases. One of the proposed mechanisms of IVIg activity is the modulation of cytokine expression and function; therefore, we analyzed the effect of IVIg on pathogen-associated molecular pattern (PAMP)-induced cytokine production by peripheral blood mononuclear cells