Staphylococcus aureus (S. aureus) is one of the most important pathogen in food poisoning and causes gastroenteritis in humans. This study was carried out to determine coagulase gene (coa gene) polymorphisms among S. aureus strains in raw milk and dairy products. A total of 320 samples of raw milk from cow, sheep and goat and 350 samples of traditional dairy products were investigated for S. aureus contamination and their genotypes of coa gene. Among the 320 raw milk samples, S. aureus were detected in 88 (27.5%) samples, and in the 350 dairy products, 87 samples (24.8%) were positive for S. aureus. S. aureus strains isolated from dairy products belong to genotype I and VIII, 45.9% and 18.3% respectively. Among S. aureus strains from raw milk samples, coa gene polymorphisms were observed only in cow milk samples and genotype I and VIII 170 Azar Asadollahi Dehkordi et al.were observed in 37.5% and 14.5% of strains, respectively. In this study only two genotypes (I and VIII) were observed among S. aureus strains in cow raw milk and dairy products in Chahar Mahal VA Bakhtiyari province, Iran. The genotype I is the most prevalent genotype among S. aureus strains in Iran.