Biochemical assessment of the body fluids gives an indication of the functional state of the various body organs and biochemical changes in the fluids which result from trypanosomiasis infections depend on the species of the parasite, its virulence, susceptibility of the host and the period of infection during which the samples are taken. Evaluations of biochemical parameters are usually done on specimen that includes serum or plasma and cerebrospinal fluid (CSF) obtain from infected animals or humans. Abnormal fluctuations are observe in indicators such as marker enzymes, electrolytes, plasma proteins, metabolites, plasma amino acids, hormones, haptoglobin, glucose, glycoproteins among other parameters on specimen collected. As observed from researchers past work, they often make use of few biochemical indicators which may be due to inability to get the appropriate ones to use at the point in time. This review is done to summarize the existing and new biochemical indicators used in trypanosome infection analysis.