Toxoplasmosis is a zoonotic protozoal disease caused by Toxoplasma gondii, an intracellular opportunistic protozoan parasite that can infect any warm-blooded vertebrate cell. In this study, zirconium, and iron-based metal-organic framework was prepared according to the solvothermal method. New nanocomposite (Curcumin@MOFs) was prepared by reacting curcumin with amino-functionalized metal-organic frameworks (Fe-MOF and UiO-66-NH 2). Besides characterizations of the composite by powder X-ray diffraction and scanning electron microscope, nano-Curcumin@MOFs was used as a new novel structure as atrial for treatment of chronic toxoplasmosis. Results showed a reduced number of brain cysts, high levels of serum Toxo IgG, and normal histo-morphology with preserved parenchymal, and stromal tissues in rats groups treated with curcumin and Curcumin@MOFs nanocomposite.

The present study investigates the effect of administration of honey bee on serum antioxidant enzymes "catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx)" and lipid profile in hypercholesterolemic male albino rats. Eighty four male albino rats weighting 150±20g were divided into six groups as follows: control group (freely supplemented with standard diet), coconut oil treated group (0.5 ml daily), cholesterol treated group (400 mg/kg b.w dissolved in 0.5 ml coconut oil), honey treated group (1 ml/rat of 20% honey bee), cholesterol and honey treated group and cholesterol before honey treated group. All treatments were by oral administration for two months. The results showed that, after one and two months coconut oil treated group showed significant increases in catalase and high density lipoprotein cholesterol (HDL-C) and significant decrease in low density lipoprotein cholesterol (LDL-C), while GPx significantly increased after two months. Treatment with cholesterol for one and two months caused significant decreases in antioxidant enzymes and significant increases in total cholesterol "TC" and LDL-C, while total lipid (TL), triglyceride (TG) and very low density lipoprotein cholesterol (VLDL-C) were significantly increased after two months. After one and two months, honey bee treated group showed significant increases in antioxidant enzymes and HDL-C and significant decreases in TG, LDL-C and VLDL-C, while TL was significantly decreased after two months. After one and two months, cholesterol and honey and cholesterol before honey treated groups showed non significant changes in antioxidant enzymes while these groups showed significant increases and decreases in HDL-C and LDL-C respectively. These results indicated that honey administration with or after cholesterol treatment acted for ameliorating the disturbances in antioxidant enzymes and lipid profile that induced by hypercholesterolemia. Honey bee administration for two months is more effective than one month in return of most measured parameters to about control values.

No abstract

No abstract

The present study was designed to investigate the effect of diazepam on the physiological changes of anxiogenic male rats. 120 male albino rats weighing 120±10g were divided into 6 groups. Control -ve (Untreated), control +ve (Treated with saline), drug (0.9mg diazepam/kg body weight), anxiety (Sleep deprived), drug+ anxiety, anxiety+ drug groups (exposed to the same conditions of drug and anxiety groups). Blood and brain samples were taken from all groups after 48 and 72 hours of anxiety. Blood was collected into two tubes, the first containing EDTA and the second without anticoagulant. Erythrocyte lysate, plasma and serum were separated and frozen at -20cº for biochemical and hormonal analysis. The results revealed that 48 and 72 hours of sleep deprivation caused depletion of SOD, CAT and GSH activities and increase of MDA levels in blood and brain of anxiogenic rats compared with control ones. The increment of plasma ACTH was also reported in anxiety group. Anxiogenic rats treated with diazepam revealed an improvement in SOD, CAT and GSH activities and depletion of MDA level in blood and brain compared with anxiety group. Plasma ACTH concentrations was decreased also in anxiogenic rats treatmed with diazepam. It might be referred that treatment with diazepam minimizing deleterious effects of sleep-deprivation induced oxidative damage suggesting its anti oxidative effect, and its inhibitory effect on the hypothalamic pituitary adrenal axis (HPA) activity.