Background: Cervical cancer is a significant health problem worldwide. It is the second most common cancer worldwide in women. Human papillomavirus types are the main cause in activating the growth of cervical cancer. This study aimed to evaluate the effect of the educational package based on health belief model on nursing students' knowledge and attitude regarding human papilloma virus and cervical cancer. Design: A quasi-experimental design (pre and posttest) was used for this study. Setting: The study was conducted in faculty of nursing at Benha University. Sample: a purposive sample included (195) female nursing students. Tools of data collection: Three main tools were used, I: A structured self-administered questionnaire which included 3 parts (1) general characteristics of student nurses,(2) medical history (3) knowledge of student nurses regarding human papilloma virus and cervical cancer , II: Modified Likert scale was developed to assess the nursing students' attitudes regarding human papilloma virus and cervical cancer and III: Health Belief Model Scale was adopted to assess the nursing students' beliefs and behaviors regarding human papilloma virus and cervical cancer. Results: There was a high statistically significant improvement among female nursing students regarding their level of knowledge, attitude and Health Belief Model about human papilloma virus and cervical cancer immediate and 4weeks post-intervention compared to pre-intervention with (P<0.001). Conclusion: Application of the educational package based on health belief model have a positive effect on improving nursing students' knowledge and attitude regarding human papilloma virus and cervical cancer. Recommendations: Application of health belief model for all university students as a healthy preventive behavior towards human papilloma virus and cervical cancer is recommended.