Background: Hand Hygiene is one of the preventive measures to reduce the health care-associated infection. Medical students spend significant time in hospital during their clinical training thus they are required to have good awareness and compliance towards hand hygiene. Aim: To assess the level of knowledge, attitude and practice on hand hygiene among medical students. Materials & Methods: An online cross-sectional study was conducted on 290 medical students who were selected through convenience sampling. The scores obtained were categorized into good, moderate and poor. Data analysis was done using Jeffreys’ Amazing Statistics Program. Result: The study showed that 58.6% of respondents have moderate knowledge towards hand hygiene, 69.3% of respondents have a good attitude towards hand hygiene and 41.4% of respondents have moderate practice towards hand hygiene. Conclusion: Educational interventions can be implemented to resolve the lack of knowledge and practice of hand hygiene among medical students. Keywords: KAP, Hand Hygiene, Medical Students, Malaysia