Background and Objective: Renal calculi or kidney stones are crystal concretions that commonly occur in the kidney and are referred to as nephrolithiasis. Ideally, calculi should form in the kidneys and pass out of the body through the urethra painlessly. Larger stones are uncomfortable and can require surgery. Numerous herbal remedies have been utilized to effectively treat urinary stones in both India and other countries. The management of ureteral stones has a variety of options. Because Musa paradisiacal has a long history of use in the treatment of nephrolithiasis. Researchers have observed the potential anti-nephrolithiatic effects of its stem juice. Hence, the purpose of this study is to determine whether plantain juice formulation (PJ), which is collected from Musa paradisiaca stems, may protect Wistar rats from ethylene glycol (EG)-induced nephrolithiasis. Materials and Methods: Rats with ethylene glycol (EG)-induced nephrolithiasis were used to test the nephroprotective effect of PJ (500 mg kgG 1 ). When EG was administered through drinking water, it caused hyperoxaluria, hypocalcemia and an increase in urea and creatinine output from the kidneys. Results: The urine excretion of calcium and oxalate was dramatically reduced with PJ supplementation. Rats given PJ had significantly lower levels of calcium and oxalate as well as less calcium oxalate crystal formations in their renal tissue than rats treated with EG. When PJ was given to rats receiving EG treatment, there was a significant decrease in creatinine, urea and uric acid. Conclusion: According to the results of this investigation, PJ supplementation prevented EG-induced nephrolithiasis by slowing the development of kidney stones. The antioxidant, diuretic and decreased amount of stone-forming elements in this substance may be the mechanism underlying this action.