Proper calculation of rice cultivation area well before harvest is critical for projecting rice yields and developing policies to assure food security. This research looks at how Remote Sensing (RS) and Geographic Information System (GIS) can be used to map rice fields in Palakkad district of Kerala. The area was delineated using three multi-temporal cloud free Sentinel-2 data with 10 m spatial resolution, matching to crop's reproductive stage during mundakan season (September-October to December-January), 2020-21. To make classification easier, the administrative boundary of district was placed over the mosaicked image. The rice acreage estimation and land use classification of the major rice tract of Palakkad district comprising five blocks was done using Iterative Self-Organisation Data Analysis Technique (ISODATA) unsupervised classification provision in ArcGIS 10.1 software, employing False Colour Composite (FCC) including Blue (B2), Green (B3), Red (B4) and Near-infrared (B8) Bands of Sentinel-2 images. The classification accuracy was determined by locating a total of 60 validation points throughout the district, comprising 30 rice and 30 non-rice points. The total estimated area was 24742.76 ha, with an average accuracy of 88.33% and kappa coefficient 0.766 in five blocks of Palakkad district. The information generated will be helpful in assessing the anticipated production as well as the water demand of the rice fields.