The composition of the yolk and albumen content in the egg was 60 and 30%, respectively. The purpose of this paper is to examine the fatty acid profile (FA) of egg yolk and albumen in cemani and white leghorn chickens. Cemani and white leghorn chickens were given a commercial feed of 110 g/head/day, and drinking water was given ad libitum. Two fresh eggs from each hen were used in this study to examine egg yolk and albumen fatty acids by gas chromatography. The data obtained were analyzed statistically with an independent sample T-test. The results showed that the FA profiles of egg yolk and albumen were not significantly different (P>0.05) for the parameters of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) from chicken. Cemani and white leghorn chickens, monounsaturated fatty acids (MUFA) are found in more amounts than SFA and PUFA in egg yolks and albumen. It can be concluded that the FA profiles of cemani and white leghorn chickens are almost the same.

<p class="abstrak2">Calf birth weight (CBW) is one of the important selection criteria to predict mature body weight and for calving ease in beef cattle. The GH and GHR genes are considered as candidate genes responsible for growth traits in cattle. The objectives of this study were to identify the polymorphism of GH|MspI and GHR|AluI genes and its association with CBW in Grati-PO cattle. A total of 186 Grati-PO cattle raised by BCRS from May to December 2017. Genomic DNA were isolated from whole blood and used in genotyping analysis using the PCR-RFLP method. The result showed that the average of CBW of Grati-PO cattle in present study was 25.58±3.31 kg. There was no statistical difference for CBW between male and female. The genotype frequency of CC, CT, and TT of GH gene were 1.1, 18.8 and 80.1 %, respectively and allele frequency of C and T of the GH gene were 0.105 and 0.895, respectively. While the genotype frequency of AA, AG, and GG of GHR gene were 66.1, 25.3 and 8.6 %, respectively, and allele frequency of A and G of GHR gene were 0.788 and 0.212, respectively. It concluded that both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-PO cattle.</p>

The growth hormone receptor (GHR) gene is member of the class I cytokine receptor family that important in growth regulation including in cattle. This study aimed to analyze the association between GHR|AluI gene with body weight, parameters in Grati-PO cattle population. A total of 304 blood samples were collected from the experimental station at the Beef Cattle Research Station, which were also equipped with 304 phenotypic data. DNA was extracted using a zymo extraction kit. Detection of of the polymorphism of GHR gene using PCR-RFLP method with AluI restriction enzymes. polymorphism indicators include genotype frequency, allele frequency, Hardy-Weinberg Equilibrium, observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC) and number of effective alleles (Ne). Association analysis was carried out using a linear mixed model. The performance of birth weight, weaning weight, yearling weight and 18 months weight were 25.4 + 0.2 kg; 113.8 + 2.3 kg; 159.9 + 2.7 kg and 213.7 + 3.6 kg, respectively. The result of association analysis was significant (P<0.05) on weaning weight, yearling weight and 18 months weight. It could be concluded that the GHR gene has potential to be a candidate genetic marker for growth traits in Grati-PO cattle.

Bali cattle is the only native Indonesian cattle that still exist and well developed. Bali cattle are known to have adaptability and a higher percentage of carcasses than other local cattle. Molecular selection related to growth traits can be done by exploration of growth hormone genes in the population of Bali cattle. This study aims to detect the level of polymorphism of GH, GHR and Pit1 genes and their association with the body weight of Grati-Bali cattle as a first step to produce a MAS (marker assisted selection). A total of 171 DNA samples Grati-Bali cattle were collected from the Beef Cattle Research Station experiment cages and its body weight data of 2014-2018 include birth weight (BW), weaning weight (WW) and yearling weight (YW). Blood samples were isolated using a zymo extraction kit. Detection of the diversity of growth hormone genes (GH, GHR and Pit1) using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with MspI, AluI, HinfI restriction enzymes. Data analysis used Chi-Square test for genotype, allele frequencies and Hardy-Weinberg Equilibrium (HWE). Association analysis used the GLM model. The results showed that the average BW, WW and YW of Grati- Bali cattle in the study were 13.3±0.3 kg, 73.7±2.4 kg and 121.3±4.2 kg, respectively and there was no statistical difference in body weight for male and female calves. The analysis showed that GH and Pit1 genes were detected by 1 genotypes respectively, each with an allele frequency > 99% and monomorphic. GHR gene was detected 3 genotypes namely AA, AG, GG, with allele frequencies A were 0.579 and G were 0.421. The GHR gene PIC value was in the moderate category. It was concluded that the GHR|AluI gene in Bali cattle is polymorphic but has not been significantly associated with the body weight of Grati-Bali cattle.

PO-Kebumen cattle was one of local genetic resources that famous with good body performance and high growth rate. The aim of this research was to detect growth hormone (GHR, GH and Pit-1) polymorphism and its association with body weight performance of PO-Kebumen cattle. The sample used in this research was 69 heads PO-Kebumen cattle belongs to the farmers of PO-Kebumen Cattle Farmer Association (ASPOKEB). This research observed the growth of PO Kebumen cattle, birth weight, weaning weight and the weight at one year. The parameters observed were birth weight, weaning weight and the weight at one year. The DNA extraction was carried out using blood samples. After DNA extraction, PCR-RFLP method was used to observe the genotype of three growth gene (GH, GHR and Pit1). The results showed that the GH and GHR genes were polymorphic, meanwhile Pit1 gene was monomorphic. The analysis showed that the three growth genes were not significantly associated to body weight (birth weight, weaning weight and the weight at one year) on PO-Kebumen cattle.