The seed infection is a result of complex factors influence: weather conditions, health conditions of used seed, quantity of infective potentila in soil, etc. By visual evaluation, initial wheat sample has been divided in four fractions: healthy, dark germed, slightly and very fusarious. Three varietes from two localities 1 and 2 have been included in analyses. Beside the wheat, the mycotoxicological contamination of flour produced by grounding of given samples was monitored, too. The representatives of genera Fusarium were dominating, and the most frequent was F. oxysporum. The wheat and flour samples have also been analysed on presence of aflatoxin B1 "AB1" and G1 "AG1", ochratoxin A "OA" and zearalenone "F-2" toxin. AG1 had the lowest representation (2,3 g/kg) and the highest representation was of F-2 toxin (even 500 g/kg)
Gas chromatography with mass spectrometry (GC-MS) was used for performing a
qualitative analysis of the hexane flour extract of three samples of spelt.
All the three samples were first treated with hexane and the obtained
extracts were used for the analysis of the fatty acid lipid components. The
transesterification reaction was performed using TMSH (trimethylsulfonium
hydroxide, 0.2M in methanol), and the fatty acids were esterified from
acylglycerol to methyl-esters. In all analyzed extracts, the predominant
component was methyl linoleate, followed by methyl oleate and methyl
palmitate. The subsequent tests, performed by cluster analysis, were used to
compare the hexane flour extracts of different types of spelt. [Projekat
Ministarstva nauke Republike Srbije, br. III46005 i br. TR 31066]
Certain climatological conditions favor the infection of wheat by field moulds, and influence very much the yield, the overall quality and the hygienic properties of the wheat. The investigations included 6 wheat varieties from the first locality and 5 varieties from the second locality. The investigated samples were most often contaminated by fungi of the genus Fusarium. Each wheat sample was classified with respect to the degree of contamination by these fungi as slightly fusarious contaminated and very fusarious contaminated grain. The mycotoxins, their metabolites, were isolated from the greatest number of contaminated grains
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