In this study, we contrast the impacts of surface coating bacterial nanocellulose small-diameter vascular grafts (BNC-SDVGs) with human albumin, fibronectin, or heparin–chitosan upon endothelialization with human saphenous vein endothelial cells (VEC) or endothelial progenitor cells (EPC) in vitro. In one scenario, coated grafts were cut into 2D circular patches for static colonization of a defined inner surface area; in another scenario, they were mounted on a customized bioreactor and subsequently perfused for cell seeding. We evaluated the colonization by emerging metabolic activity and the preservation of endothelial functionality by water soluble tetrazolium salts (WST-1), acetylated low-density lipoprotein (AcLDL) uptake assays, and immune fluorescence staining. Uncoated BNC scaffolds served as controls. The fibronectin coating significantly promoted adhesion and growth of VECs and EPCs, while albumin only promoted adhesion of VECs, but here, the cells were functionally impaired as indicated by missing AcLDL uptake. The heparin–chitosan coating led to significantly improved adhesion of EPCs, but not VECs. In summary, both fibronectin and heparin–chitosan coatings could beneficially impact the endothelialization of BNC-SDVGs and might therefore represent promising approaches to help improve the longevity and reduce the thrombogenicity of BNC-SDVGs in the future.
Radio frequency plasma power dependence of the moisture permeation barrier characteristics of Al2O3 films deposited by remote plasma atomic layer deposition Comparison between ZnO films grown by plasma-assisted atomic layer deposition using H2O plasma and O2 plasma as oxidant J. Vac. Sci. Technol. A 31, 01A142 (2013); 10.1116/1.4771666Substrate-biasing during plasma-assisted atomic layer deposition to tailor metal-oxide thin film growth J. Vac. Sci. Technol. A 31, 01A106 (2013); 10.1116/1.4756906Reaction mechanisms during plasma-assisted atomic layer deposition of metal oxides: A case study for Al 2 O 3Plasma-assisted atomic layer deposition (PALD) was carried for growing thin boron oxide films onto silicon aiming at the formation of dopant sources for shallow boron doping of silicon by rapid thermal annealing (RTA). A remote capacitively coupled plasma source powered by GaN microwave oscillators was used for generating oxygen plasma in the PALD process with tris(dimethylamido)borane as boron containing precursor. ALD type growth was obtained; growth per cycle was highest with 0.13 nm at room temperature and decreased with higher temperature. The as-deposited films were highly unstable in ambient air and could be protected by capping with in-situ PALD grown antimony oxide films. After 16 weeks of storage in air, degradation of the film stack was observed in an electron microscope. The instability of the boron oxide, caused by moisture uptake, suggests the application of this film for testing moisture barrier properties of capping materials particularly for those grown by ALD. Boron doping of silicon was demonstrated using the uncapped PALD B 2 O 3 films for RTA processes without exposing them to air. The boron concentration in the silicon could be varied depending on the source layer thickness for very thin films, which favors the application of ALD for semiconductor doping processes.
The pressure dependence of the deposition rate for magnetron sputter deposition of various elemental semiconductors and metals was investigated by x-ray measurements on sputtered films and quartz monitor measurements. It was found that for all elements investigated the dependence of the rate Φ on pressure-distance (pd) is well described by Φ=Φ0(1−e−cpd)/cpd. The value of c equals the inverse characteristic pressure-distance product (pd)0, which is the characteristics of the exponential decay of rate with pressure for low pressures. The experimental data of (pd)0 vary from 4.6 Pa cm for aluminum to 120 Pa cm for tungsten. It is shown that (pd)0 depend on both material specific properties and process parameters. The material specific properties are mainly the atomic mass and diameter, and the surface binding energy. The process parameters target voltage and power density act via the increase of the mean free path and the reduction of gas density, respectively, on (pd)0. As a first approximation, the characteristic pressure-distance product for argon as sputtering gas is proportional to the product of target atomic mass, average atomic energy and thermal mean free path.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.