Since bygone times natural remedies remain a primal source of medicine for public health. Triphala and cow urine are the inherent natural remedies recommended in the time-honored medicinal system of Ayurveda. Traditionally cow urine is practiced with multifarious plant species for differential medicinal values but still unnoticed by the scientific world for the investigation of its traditional assert. The present appraisal deals with the evaluation of antioxidative and enzyme inhibitory strength of cow urine extract of Triphala by in vitro methods. The antioxidative capability of the cow urine extract was noteworthy in DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical curbing mode, successively delineated the IC50 statistics of 95.47 μg/ml and 138.92 μg/ml, respectively. The antidiabetic competence was examined through the approach of inhibitory action averse to α-amylase and α-glucosidase serially rendered the IC50 statistics of 172.97 μg/ml and 199.56 μg/ml while the accomplishment of anti-inflammatory prospects against lipoxygenase was beheld with the IC50 statistics of 123.57 μg/ml. The cow urine extract expressed a substandard rendition of antiproteinase scrutiny. In the course of experimentation, traditional claims of herbal treatment in combination with cow urine may become instrumental to curb the cultivation of distinct diseases and beneficial outcomes of cow urine extract of Triphala are presumably linked to the essential bioactive chemical substances in the extract.
Plant species always remain a backbone for the discovery of active biomolecules required for public health. The existing scrutiny is outlined to dissect the chemical nature and explore the antioxidant and anti-inflammatory strength of methanol extract of aerial parts of Lindernia antipoda by in vitro methods. The basic phytochemical evaluation of the extract reflected the existence of essential nitrogenous and phenolic bio-compounds. UV-visible and FTIR (Fourier Transform-Infrared Spectroscopy) spectral analysis supported the chemical nature of reflected compounds. The antioxidant capability was noticed through nitric oxide scavenging assay and ABTS [2,2’-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid)] radical cation scavenging assay while anti-inflammatory competence was examined through the stabilization approach of human erythrocytes membrane against heat-induced membrane lysis. The methanol extract showed nitric oxide scavenging and ABTS radical cation scavenging by IC50 values of 398.38 μg/mL and 513.30 μg/mL respectively while anti-inflammatory activity was observed with the IC50 value of 573.01 μg/mL. The outcomes of each activity of methanol extract are presumably linked to the essential bioactive chemical substances in the extract
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