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Internal transcribed spacer (ITS) 86F and ITS4 and the ITS1-F and ITS86R primer pairs were tested to specifically amplify fungal community DNA extracted from soil. Libraries were constructed from PCR-amplified fragments, sequenced and compared against sequences deposited in GenBank. The results confirmed that the ITS86F and ITS4 primer pair was selectively specific for the Ascomycetes, Basidiomycetes and Zygomycetes fungal clades. Amplified products generated by the ITS1F and ITS86R primer pair also aligned with sequences from a range of species within the Ascomycete and Basidiomycete clades but not from the Zygomycete. Both primer sets demonstrated fungal specificity and appear to be well suited for rapid PCR-based (fingerprinting) analysis of environmental fungal community DNA. This is the first reported use and assessment of the ITS86F and ITS4 and the ITS1-F and ITS86R primer pairs in amplifying fungal community DNA from soil.

A glasshouse experiment was carried out to assess the feasibility of applying partial rootzone drying (PRD) to highbush blueberries (Vaccinium corymbosum). A subsequent field experiment was established to assess four irrigation strategies with the aim of improving water use efficiency in blueberry production. Applying PRD to plants during a glasshouse experiment reduced stomatal conductance without reducing plant water potential. Hindered by high rainfall, a physiological response to PRD was not repeated in field grown plants. However, irrigation scheduled using a K c (crop coefficient) curve constructed from Food and Agriculture Organization (FAO) 56 guidelines and post-harvest regulated deficit irrigation (RDI) delivered annual water savings of 0.8 ML ha(1 and 1.3 ML ha (1 , respectively, compared with a total 3.6 ML ha(1 applied using a 'rule-of-thumb' approach commonly adopted by Australian blueberry growers. These savings were achieved without reducing berry yield or quality. This study is the first to report on the feasibility of applying FAO 56 guidelines, RDI and PRD as strategies to maximize water use efficiency in highbush blueberry production.

A non-destructive, simple and accurate method of determining the relative growth rate (RGR) of the packed cell volume (PCV) of plant suspension cells in one Erlenmeyer flask at any time during the incubation period is described. The Erlenmeyer flask was tilted and the length of the chord formed by the surface of the packed cells across the bottom of the flask was measured. The chord length and the log PCV were correlated in a calibration line. The method enables the RGR during the exponential growth phase to be calculated by multiplying the slope of the linear part of the curve of the chord length in time with the slope of the calibration line. In order to investigate other growth parameters and to analyse the accuracy of the method statistically, a four-parameter function for the chord length and a computer program were used.The RGR during the exponential growth phase of cell suspensions of Solanum tuberosum and Haplopappus gracilis appeared to be independent of the PCV of the inoculum. The method appeared to be sufficiently accurate.

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