Oxygen permeability coefficients (OP), water vapor permeability coefficients (WVP), ethylene permeability coefficients (EP), tensile strength (TS) and percent elongation (%E) at break values were determined for chitosan films plasticized with glycerin at two concentrations (0.25 and 0.50 mL/g chitosan). Film samples were tested after 0, 2, 4, 8 and 12 wk of storage. After an initial drop in permeability during the first 2 wk of storage, mean OP (4.6 ϫ 10 Ϫ5 cc/m⅐day⅐atm) and mean EP (2.3 ϫ 10 Ϫ4 cc⅐/m⅐day⅐atm) remained constant while mean WVP (2.2 ϫ 10 Ϫ1 g/m⅐day⅐atm) decreased with respect to storage time. TS values (15-30 MPa) decreased and %E values (25%-45%) increased with respect to storage time. The stability of OP and EP values with storage was not expected, while the change in mechanical properties was as expected.