Biological aspects and global demand for aquarium promote seahorses as new species with high potential for commercial purposes; however, the low newborn survival rate represents the main bottleneck of seahorses farming. In this study, the organogenesis of the Hippocampus reidi was analysed from release until the 30th day after birth, using histological and histochemical approaches. To study the stages of their early life, 360 individuals were killed, sectioned, and stained with haematoxylin and eosin, periodic acid-Schiff, and Sudan Black B techniques. At birth, mouth and anus were open, the swim bladder inflated, and the visual system highly developed. Among the results, it was emphasized the presence of the yolk sac until the 2nd day after birth, the loops of the intestine to accommodate its elongation, and the ability of the larvae to absorb lipids in the anterior and posterior tract of the intestine. A short time (7/8 days) between reabsorption of yolk sac and formation of gonads was registered, with primordial follicles visible from the 10th day after birth. For the first time, organogenesis in H. reidi was described in detail; seahorses underwent a marked metamorphosis, and the indirect development observed in this species lead up to reconsider the term "juvenile" used for H. reidi during this period.
Little is known about the nutritional requirements of ornamental fish and the effects which they may have on fish histology. Because of this, 45 young, captive-bred seahorses (1.37 g AE 0.51) were randomly divided and put into fifteen, 35-L, glass aquariums. Three experimental diets were tested in triplicate: live enriched Artemia (LEA); frozen enriched Artemia (FEA); and commercial Mysis (M). seahorses were fed twice daily, 6 days a week for 102 days throughout the experiment. The body weight and length of the seahorses were measured every 20 days, and at the end of the experiment, fish liver samples were taken for histological studies. Biochemical analyses of the diets and the seahorses fed on the experimental diets were recorded, and the differences in the total lipid content in each of the three diets were also observed, each reflecting their respective liver lipid content. seahorses fed with LEA treatment showed lipid vacuoles in the hepatocytes with nuclei displaced to the periphery. Similar results were also observed in FEA fed seahorses, but to a lesser extent. In contrast, the M fed seahorses were characterized by having livers with lower size hepatocytes and centrally located nuclei.
Information about early development after male release lags behind studies of juveniles and adult seahorses, and newborn seahorses, similar in shape to adults, are considered juveniles or fry. During early life, Hippocampus hippocampus present behavioural (shift in habitat, from planktonic to benthic) and morphological changes; for this reasons, the aims of this study are to define the stage of development of H. hippocampus after they are expelled from the male brood pouch and to establish direct or indirect development through an osteological analysis. The ossification process was studied in 120 individuals, from their release to 30 days after birth. To analyse the osteological development, Alcian Blue-Alizarin Red double staining technique for bone and cartilage was adapted to this species. At birth, H. hippocampus presents a mainly cartilaginous structure that ossifies in approximately 1 month. The bony armour composed of bony rings and plates develops in 10 days. The caudal fin, a structure absent in juveniles and adult seahorses, is present at birth and progressively disappears with age. The absence of adult osteological structure in newborns, like coronet, bony rings and plates, head spines and components allowing tail prehensile abilities, suggests a metamorphosis before the juvenile stage. During the indirect development, the metamorphic stage started inside brood pouch and followed outside and leads up to reconsider the status of H. hippocampus newborns.
Fertilized and unfertilized eggs and embryos of the longsnout seahorse Hippocampus reidi were collected at different stages of development and provided the basis for a description of morphological development from fertilization until release from the paternal pouch. Images of fertilized eggs, as well as their rupture after a few minutes in seawater are reported for the first time. The yolk sac transitioned from ovoid to spherical shape and was reabsorbed progressively until release. The tail began rising from the surface of the deuteroplasm while embryos were in the egg envelope. Embryos lacked a primordial fin fold and developed some species characteristics, such as rays in the dorsal fin, before resorption of the yolk sac. At release, juvenile seahorses were in an advanced stage of development even if they lacked important adult characteristics, such as ring plates and coronet. The tail was not prehensile in juveniles at release; a small caudal fin was present, although this fin is lost in adults.
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