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A Stability indicating isocratic liquid chromatographic method with UV detection at 255 nm is described for simultaneous determination of sofosbuvir and velpatasvir in its bulk and tablet dosage form. Chromatographic separation of two drugs was achieved on a YMC column (4.6×15mm,5 ) using a mobile phase consisting of a binary mixture of acetonitrile and 0.025M KH 2 PO 4 adjusted to pH3..0 with orthophosphoric acid in ratio 50:50. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. Linearity, accuracy and precision were found to be acceptable over the concentration range of 50-250 µg/ml for velpatasvir and 200-1000µg/ml for sofosbuvir and R 2 found to be 0.999. Accuracy was measured via recovery studies and found to be acceptable, and the percentage recoveries were found in the range of 97-103%.Method precision results obtained are 0.1%RSD for sofosbuvir and 0.8%RSD for velpatasvir. Forced degradation studies were also conducted, and the drugs were subjected to various stress conditions such as acid hydrolysis, base hydrolysis, oxidative, photolytic and thermal degradation. The proposed method was successfully validated and applied for the quantitative estimation of these drugs in both bulk and tablet dosage forms. The LC method can be used for the quality control of formulated products containing sofosbuvir and velpatasvir.

Chitosan has become a prominent polymer attracting a lot of interest. The fact that this precious polymer may be recovered from shell waste created by the food industry adds to its worth. Because of its biological and physicochemical characteristics, chitosan has become a popular choice for biomedical applications. The different processes involved in the production of chitosan and chitosan Nano forms are briefly discussed in this article. We bring together for the first time, studies on diverse initiatives at green chitosan production, chitosan nanoparticles, and chitosan Nano composites. Chitosan aids in the production of more stable nanoparticles. The formation of nanoparticles was confirmed by UV-Visible spectroscopy, which detected a peak at 529 nm. Based on the interpretation of the spectroscopy in this study, the interaction between chitosan and generated gold nanoparticles (AuNPs) was studied and assessed, and it can be concluded that FT-IR spectroscopy was utilized to analyze all functional groups. XRD examination confirmed the presence of gold nanoparticles with crystalline nature, which were discovered to have an FCC lattice structure. Atomic force microscopy (AFM) is a technique for analyzing the surface of a rigid material all the way down to the level of the atom. AFM uses a mechanical probe to magnify surface features up to 100,000,000 times, and it produces 3-D images of the surface. The potency of the synthesized AuNPs against the selected fungi increases with increase in concentration ofAuNPs. Therefore, the antifungal investigation revealed that the synthesized AuNPs exhibited good antimicrobial activity.