A B S T R A C TCurcuma karnatakensis, a member of Zingiberaceae, is endemic to the state of Karnataka, India. The structure and physicochemical properties of starch isolated from rhizomatous rootstocks of two samples -A and B were analyzed for the first time. Sample A contains 76.4 AE 0.3% of starch, of which 86.6 AE 0.4% is amylose, while sample B has 75.0 AE 0.4% of starch containing 84.6 AE 0.4% of amylose according to UV-Vis spectrophotometric analysis. The shape of the starch granules in both the samples is polygonal and cuboidal with a smooth surface, as revealed by SEM studies. The X-ray diffractogram indicated A type of polymorphs in contrast to other Curcuma species, where B types are reported. Since its high amylose content leads to an increased tendency to retrogradation and the formation of resistant starch, this taxon could become one of the major dietary sources of starch in the future. In addition, a source rich in amylose specifies its prospective application in the pharmaceutical and biodegradable film industry.