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The use of Annona muricata (A. muricata) in the treatment of various human pathologies is becoming more and more widespread in Benin. The aim of this study was to inventory the diseases treated by the use of this plant. For this purpose, an ethnobotanical survey was carried out among 77 herbalists and 170 traditional therapists from the Departments of Ouémé and Plateau, Littoral, Atlantic and Mono. The data collected was relative, to the ecological were usually the fruit (86.2%), the leaf (66.4%) and the bark (62.8%). According to the actors, Annona muricata was mainly used for the treatment of insomnia (70.9%), cancer (70%), malaria (59.9%), high blood pressure (58.7%), vertigo (57.5), etc. The main method of organ preparation was herbal tea (97.2%), decoction (93.5%), and powder (76.9%). Therefore, at the results showed, this study allowed to know ethnobotanic value of Annona muricata.