Aira capillaris Host var. ambig~a Not. Strandja, an Felsen bei dem Dorfe Kosti. Poa caesia Sin. An Felsen bei Sara-Gjol (Rila) und Resnovete (Vito~a). _Festuea Lachenalii Spenn. var. mutica Asch. et Graebn. Strandja, an Felsen bei Kosti. Scirpus triqueter L. Auf Sand am Meeresstrande bei Mesemvria. Schoenus nigrieans L. Auf Sand am Meeresstrande bei Burgas. Carex prolisa Fries. An dem Sumpfe bei Dragoman. Allium nigrum L. Auf Ackerfeldern bei Nova Machla (Bezirk Philippopel), gesammelt von St~ibrny. Bis jetzt nur am Ali-Botu~ in Ost-Mazedonien gefunden.-Cyrilli Ten. Am Rande der Ackerfelder bei dem Dorfe Poturnak (Strandja). Nectaroscordum siculum Ucria vat. Dioscoridis Rieht. In schattigen Wiildern bei Kiiprija (Strandja). Innerhalb der Grenzen Bulgariens nur ffir die Umgebung yon Varna bekannt (Davidov). 1"ulipa australis Lev. An den Hiigeln Bakardjik bei Jambol. Diese Art scheint in Bulgarien ziemlich welt verbreitet zu sein, ~ wird aber iifters mit T. silvestris verwechselt, zu der sie auch ()bergangsformen bildet. Scilla bithynica Boiss. (S. l~adkae Davidov, in Magyar Botanikai Lapok, 1905.) In schattigen Uferwiildern an dem Flusse Kargana, sfidlieh yon Sozopol. Ornithogalum Wiedemanni Boiss. An dem Strandja-Gebirge, in Gebilsehen bei Malko-Tirnovo und Siva. Gladiolus paluster Gaud. Ali-Botu~, an grasigen Stellen auf (~engene-Kale (1300 m). Orchis provincialis Balb. Strandja, in Gebiischen bei Kosti und nSrdlich yon Pergopla. Salix retusa L. Auf Felsen des Gipfels Ibar (2500 m) an dem Rila-Gebirge und auf'dem Sinanica-Gipfel an dem Pirin. Moenchia quaternella Ehrh. An feuchtem Lehmboden bei Poda, stidlich yon Burgas; selten. Arenaria graeca Boiss. Auf Felsen in der Schlucht Jundol (Rhodopen). Anemone pavonina Lam. (A. fulgens Gay) typica. In Gebtlschen und liehten Wifldern bei Kiiprija und Siva (Strandja).

A novel thermostable MnSOD was purified to electrophoretic homogeneity from the fungal strain Humicola lutea 110. The preparation of the pure metalloenzyme was performed using treatment with acetone followed by ion exchange and gel permeation chromatography. We found that the activity of this enzyme comprises about 80% of the total superoxide dismutase activity in the crude extract, containing two proteins: MnSOD and Cu/ZnSOD. The MnSOD has a molecular mass of approximately 76 kDa and 7200 U/mg protein specific activity. It is a tetrameric enzyme with four identical subunits of 18 860 Da each as indicated by SDS-PAGE, amino acid analysis and mass spectrometry. N-terminal sequence analysis of MnSOD from the fungal strain revealed a high degree of structural homology with enzymes from other eukaryotic sources. Physicochemical properties were determined by absorption spectroscopy and circular dichroism measurements. The UV absorption spectrum was typical for an MnSOD enzyme, but displayed an increased absorption in the 280 nm region (epsilon280 = 10.4 mM(-1). cm(-1)), attributed to aromatic amino acid residues. The CD data show that MnSOD has two negative Cotton effects at 208 and 222 nm allowing the calculation of its helical content. The ellipticity at 222 nm is 6800 deg. x m(2) x dmol(-1) and thus similar to the values reported for other MnSODs. The MnSOD from H. lutea 110 is stable over a wide range of pH (4.5-8), even in the presence of EDTA. The enzyme is thermostable at 70-75 degrees C, and more stable than MnSODs from other sources.

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The well known inhibitory effect of abscisic acid (ABA) on growth and protein mobilization was confirmed. In general benzylaminopurine (BAP) is known as a stimulator of plant growth. However in very high concentration BAP provoked a little suppression of plant growth and of activity of some proteolytic enzymes. Irrespective of this a faster hydrolysis of storage proteins in BAP treated seeds was detected. Similar results to BAP action after N 1 -(2-chloro-4-pyridyl)-N 2 phenylurea (4PU-30) treatment were obtained. Data for growth, proteolytic activity and protein content of maize seedlings treated with in an identical high concentration enlarged our knowledge for their relationship and supported the assumption for antagonistic action of these agents with very strong structural similarity. As CS is newly developed and widely used herbicide these results have not only theoretical but also practical significance. Abbreviations:ABA