Introduction Measured total carbon dioxide (TCO2) from venous sample and calculated bicarbonate from arterial blood gas (ABG) have shown good agreement in some studies, while conflicting results have been obtained in few other studies. The objective of this study is to compare and assess the degree of agreement between the measured TCO2 and calculated bicarbonate and also whether they can be used interchangeably in our laboratory. Materials and methods We prospectively analyzed 89 ABG samples requested for calculated bicarbonate and then measured TCO2 from venous blood samples drawn simultaneously from the same participants between November 2016 and April 2017. Results Measured TCO2 results ranged from 5.7 to 39.9 mmol/L (mean 23.45 mmol/L), while calculated bicarbonate ranged from 9 to 40 mmol/L (mean 24.36 mmol/L). The values of TCO2 and bicarbonate correlated well (r = 0.95, p < 0.001), with the correlation given by the equation, y = 0.884x + 3.605. The bias obtained was —0.9 mmol/L and the standard deviation (SD) was 1.62 mmol/L. The limits of agreement (LOA) were —4.1 to 2.3 mmol/L, with a span of 6.4 mmol/L. Out of the 89 values, 85 (95.05%) were within the LOA. Conclusion In majority of the cases, the calculated bicarbonate concentration from ABG showed a good correlation to the measured venous TCO2 concentration. Despite this excellent correlation, TCO2 did not show good agreement with calculated bicarbonate when Story and Poustie's criteria were applied, especially in cases of bicarbonate less than 20 mmol/L. Hence, clinicians should be aware of this discrepancy and be cautious when using measured TCO2 and calculated bicarbonate interchangeably in the assessment and management of acid—base disorders, especially in patients with metabolic acidosis. How to cite this article Mohan T, Kumar BV. Comparison of measured Serum Total Carbon Dioxide with calculated Bicarbonate calculated from Arterial Blood Gas Analysis. Indian J Med Biochem 2017;21(2):76-80.

Aim of the study role of acid base balance in chronic severe anaemic patients admitted in Department of Medicine, Darbhanga Medical College and Hospital, Laheriasarai, Bihar. Study subject include 50 chronic and severe anaemic patients. All the fty cases underwent detailed haematological, biochemical, electrocardiographic, roentgenographic studies followed by extensive analysis of blood for blood gases and acid base parameters at the onset. Some were further studied for venous blood lactate and the effect of intravenous frusemide on the acid base parameters and the clinical state of the patient. Twenty normal controls were also studied. Work in future on the problem of chronic severe anaemia should include cases with cardiac failure and other complications like respiratory infections etc. and they should be studied for myocardial function, glomerular ltration rate and renal anion transport in addition to blood gases and acid base study as done by us. The role of ionotropic agents should be studied in such cases in isolation from that with diuretics. The administration of oxygen alone with a mask is also expected to improve almost all parameters quickly and should be studied.