Clinoptilolite mineral which is a member of zeolite family has been recently used in medical and dental applications. Until today, it has never been used as a graft material. In the present study tricalcium phosphate (TCP) imbued 90-95% pure clinoptilolite was used as graft in rabbit tibia. General anesthesia was accomplished using intra muscular (IM) injections of Xylazin and Ketamine HCL. TCP imbued clinoptilolite was placed in the defects created in the tibias of the subjects. Control group defects were left empty. One group was sacrificed on day 28 and the other group on day 56 to evaluate osteogenesis, residual graft material, inflammation and fibrosis. Histological evaluation revealed new bone formation at 28 days to be 14/7 at control group where as its 11/7 for the experimental graft group. At 56 days the values are as 18/7 for control and 14/7 for the experimental graft groups (n = 7). Our study group is the first to perform intrabony application of clinoptilolite. No evidence of abnormal inflammatory cell formation or fibrosis was witnessed in groups. When TCP, which can be used as a standalone graft material, was mixed with zeolite it was not as effective as expected. We believe that, TCP particles are absorbed and captivated in the initial phase and due to absorption strength of zeolite cannot be released back to the environment. We assume its effect can increase with longer time periods. Bone formation without infection is observed around graft particles. Based on the present study, since clinoptilolite does not provoke an inflammatory process, its use in unison with TCP can provide a supporting structure in defects.

Rough surfaces of denture prosthesis promote adhesion of microorganisms and plaque formation. In dentistry ferric oxide, tin oxide, chrome oxide and pumice are the most commonly used materials for polishing of resins and other materials. Clinoptilolite that is in zeolitic structure resembles to pumice due to alumina silicate formations in its constitution. Surface roughness was evaluated by means of a surface roughness analyzing instrument, Perthometer There were 10 specimens for each acrylic resin and bis-acryl composite resin material and polishing method combination. Conventional lathe polishing with polishing paste served as surface roughness was determined with a profilometer and SEM microscope. ANOVA was applied to the all measurements and was statistically significant (p<0.001). The p-values were calculated using paired t-tests. The materials in order that are most successful at polishing PMMA surface are; clinoptilolite Ra 0,5 > Fe2O3 Ra 0,6 > Cr2O3 Ra 0,8> pumice Ra 1,2 > SnO2 Ra 1,6. the surface roughness values for Bis-acryl Composite are as follows: clinoptilolite Ra 0,6 > Fe2O3 0,8 Ra > Cr2O3 Ra 0,9 > pumice Ra 1,4 > SnO2 Ra 1,9. The most successful material for PMMA and Composite resin were clinoptilolite followed by; clinoptilolite, Fe2O3, Cr2O3, pumice, and SnO2 in order. The same results were confirmed with the scanning electron microscopic images. Within the light of the present study Clinoptilolite can be an alternative to pumice for polishing of PMMA.