Root extracts of the plant Abrus precatorius L. was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography. The antibacterial activity was localized to specific chromatophores in the chloroform fraction through a bioautography assay. It was found localized to 4 chromatophores out of 7. The chromatophores were isolated from the TLC plates and rechecked for activity against Staphylococcus aureus A, using a disc diffusion assay. Among the four active principles isolated, AP 3 (Rf 0.87) exhibited maximum activity, i.e., 56% inhibition of growth of S. aureus A, in disc diffusion assay compared to the standard antibiotic Ampicillin. Results of this study suggest that chloroform extractable phytochemicals in A. precatorius L. may yield promising molecules with antibiotic activity.
Root extracts of the plant Abrus precatorius L., was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography (TLC). The antibacterial activity was localized to specific chromatophores in the chloroform fraction through a bioautography assay. It was found localized to four chromatophores out of seven. The chromatophores were isolated from the TLC plates and rechecked for activity against Slaphylococcus aureus A, using a disc diffusion assay. Among the four active principles isolated, AP 3 (Rf 0.87) exhibited maximum activity i.e. 56% inhibition of growth of S. aureus A, in disc diffusion assay compared to the standard antibiotic Ampicillin. Results of this study suggest that chloroform extractable phytochemicals in A. precatorius L. may yield promising molecules with antibiotic activity.
Vanillin, a major component of the vanilla is known to exhibit various antimicrobial properties including inhibition of bacterial biofilms. However, a systematic study on activities of vanillin against the most common fungal pathogen, Candida albicans, is lacking. In this study, broth micro dilution method was used to assess vanillin's activity on the planktonic growth of C. albicans. Minimum inhibitory concentration (MIC) was established at 1 mg ml -1 of vanillin. Prevention of yeast to hyphae morphogenesis was evident at 500 μg ml -1 of vanillin, in standard micro plate based assay. Adhesion, biofilm development and mature biofilms were studied in an in vitro biofilm model using XTT-metabolic assay and light microscopy. Significant (p < 0.05) reduction in biofilm development was observed in presence of 500 μg ml -1 vanillin. Concentration dependent decrease of cellular ergosterol in presence of vanillin indicated that inhibition of ergosterol biosynthesis may be a probable target. Our study, for the first time reveals vanillin as a potential inhibitor of biofilm formation by C. albicans.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.