The modified low-density lipoprotein (LDL; glycated and oxidized) is more atherogenic and are poorly recognized by LDL receptor. In the present study, an attempt has been made to evaluate the effects of Triphala on LDL modification in experimentally treated rats and in human LDL. The effect of ethanolic extract of Triphala on LDL oxidation susceptibility in high fat diet fed and STZ induced rats was evaluated .The inhibitory effect of Triphala on LDL oxidation and glycation was investigated using copper and glucose induced human LDL, respectively in vitro. The co-presence of Triphala extract significantly increased lag phase and minimized the conjugated dienes formation in dose dependent manner. Copper mediated LDL oxidation was characterized by elevated indices of thiobarbituric acid reactive substance (TBARS), whereas co-presence of Triphala extract significantly minimized the production of TBARS. The results of this investigation shows that Triphala probably with their antioxidant properties inhibited LDL glycation and oxidation and provide scientific reasons regarding the possible medical benefits of using Triphala to prevent diabetic and cardiovascular complications.