Serpentine soil contains highly heavy metals, such as manganese, chromium, cobalt, and nickel,which could bean inappropriate growthmediaofmostplants. Someplants thatfound able to grow optimally on South Kalimantan serpentine soil have been known to do association with ectomycorrhizal fungi. This research aimed to obtain and characterize mushrooms assumed as ectomycorrhizal fungi indigenous South Kalimantan serpentine soil. This study used field exploration of fungal fruiting bodies and identified the genus based on morphological characters of fruiting bodies such as shape, size, and ornamentation, which are unique for the genus identification, then compared the characteristics on mushroomexpert.com. The mushrooms were also confirmed of genera assumed as ectomycorrhizal fungi based on mycorrhizas.info. Seven fruiting bodies were obtained and classified as Cantharellus (Ct), Chlorophyllum (Ch1 and Ch2), Lycoperdon (Ly), Ramaria (Rm1 and Rm2), and Thelephora (Tp). The results showed that all of those fruiting bodies belong to Basidiomycetes. There were 4 genera of Cantharellus, Lycoperdon, Ramaria, and Thelephora, assumed as ectomycorrhizal fungi. But Chlorophyllum genus was never reported as ectomycorrhizal fungus

Phylogenetic analysis of mangoes species using the nucleotide sequences of 5.8S rRNA genes aimed to determine the cladistics and evolution of kinship amongst the species of the genus Mangifera to support the conservation and breeding efforts of local endemics of South Kalimantan. The analysis involved 23 nucleotide sequences of the 5.8S rRNA gene obtained from the GeneBank of NCBI which were aligned and trimmed into 655 bp using MEGA X software. The Kimura-2 parameter model and  Maximum-Likelihood method used to construct a monophyletic tree of 4 clades. Clade I consists of M. torquenda, M. sumatrana, M. kemanga, M. quadrifida, M. griffithii, M. casturi, M. lalijiwa, M. macrocarpa, M. flava, M. gracilipes, M. pentandra and M. caloneura. Clade II consists of M. foetida, M. cochinchinensis, M. gedebe, M. oblongifolia, M. indica, M. laurina, M. sylvatica, and M. zeylanica. Clade III only consists of M. andamanica. Clade IV consists of M. odorata and M. camptosperma. The closest kinship occurred between M. odorata and M. camptosperma and the farthest kinship was M. camptosperma. M. andamanica has the farthest evolutionary distance with the longest branch.

Improving the achievement of competencies which are indicators in the study of the quality of education in Indonesia, can be done by procuring quality and innovative teaching materials. This study aims to develop a science learning handout on the human digestive system to improve critical thinking skills for class VIII SMP. Development is done by (Educational Design Research). This study aims to develop a science learning handout on the human digestive system to improve critical thinking skills for class VIII SMP. This article describes the results of the validity and practicality of science learning handouts on the human digestive system. The instrument used in this study was a validity and practicality questionnaire. Data analysis techniques include descriptive data analysis, validation sheets, and questionnaires. The handout validation sheet is given to 5 validators. Instead, 31 grade VIII students of SMP Negeri 5 Banjarmasin were given practice sheets. The results of the validity questionnaire analysis concluded that the validity of the handout was 3.67 very valid categories. Practicality test of content average of 87.41 was obtained in the very practical category. The results of the practicality test of expectations average of 87.78 in the very practical category. Finally, the actual practicality test results average of 85.65 with a very practical category. This means that science handouts are valid and practical in the learning process to practice critical thinking skills

Abstract. Mursyidin DH, Nazari YA, Badruzsaufari, Masmitra MRD. 2021. DNA barcoding of the tidal swamp rice (Oryza sativa) landraces from South Kalimantan, Indonesia. Biodiversitas 22: 1593-1599. The tidal swamp rice (Oryza sativa L.) landraces of the South Kalimantan, Indonesia, has been known for hundred years ago with a better adaptation to the local conditions, such as acidity, salinity, and metals contamination. However, the genetic insight of these landraces has not fully understood. Here, the rbcL region of tidal swamp rice from this region was successfully sequenced, aligned, analyzed, and deposited into the GenBank with accession numbers of MT818188–MT818201. The multiple alignments of these sequences showing a barcoding motif with eight mutation or polymorphic sites with both substitutions (transition-transversion) and indels (insertion-deletion). Based on this marker, the tidal swamp rice has a low genetic diversity, only 0.086. However, the UPGMA and maximum likelihood (ML) analyses revealed that this germplasm was grouped into five and twoclusters or clades, respectively. In this case, Bayar Putih is closely related to Siam Panangah and farthest from Lemo. This information might help to develop conservation and breeding programs of the tidal swamp rice landraces in the future.

Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Periphthalmodon schlosseri, Pallas 1770) is one of important biological diversity that potential as bio-indicator in environment assessment which is heavy metal contamination. These fish are susceptible to ambient environment, that effect to their physiological mechanism including protein synthesis and expression. This research aims to identify protein profiles of giant mudskipper muscle and review its potential application as a biomarker for heavy metal contamination in Barito Estuary of South Kalimantan. Total proteins were isolated from fish muscle using Tris EDTA buffer and then precipitated using Ammonium sulfate salt. The protein concentration was measured using Bradford assay and then separated based on molecular weight using the SDS-PAGE method. The result showed muscle proteins of giant mudskipper could be precipitated on optimum condition at Ammonium sulfate saturation 60-70% which protein concentration 5.106 mg.mL-1. The protein separated into 20 bands for raw and precipitated proteins which molecular weight ranging from 33 into 184 kDa. Further study is needed for protein identification based on amino acids sequence of protein bands for biomarker discovery and validation.