Background: One of most common surgical technique applied overall the world is laparoscopic cholecystectomy due to the high features instead of another surgical techniques. Furthermore; it is represents as the main technique for gallstones treatment; however the incidence of bile-duct injury still higher than that of open cholecystectomy. Various surgical procedures and principles have been proposed to reduces bile duct injury. Aim of the work:To determine the frequency and the type of Rouviere's sulcus and its importance as landmark during laparoscopic cholecystectomy. Patients and methods: This prospective study included hundredpatients who had symptomatic gallstone disease and underwent laparoscopic cholecystectomy in Al Azhar Universty Hospitals and Ahmed Maher Hospital. Results: Our results revealed that, regarding the indications for cholecystectomy, the most common indication in the study participants were symptomatic gall stones in 36 (36%) of participants followed by acute cholecystitis in 29 (29%) participants and biliary pancreatitis in 16 (16%) participants. Conclusion: Rouviere's sulcus was re-present in (76%) of total cases. It is an easily identifiable anatomic feature for secure laparoscopic cholecystectomy.