In the present work, the oxidation of acetaminophen in the absence and presence of eflornithine was electrochemically investigated by means of cyclic voltammetry at a glassy carbon electrode (GCE). Our results indicate that N‐acetyl‐p‐benzoquinone imine (NAPQI) produced from two‐electron electrochemical oxidation of acetaminophen participates in a Michael addition reaction with eflornithine via an ECE mechanism. This fact was used for the determination of eflornithine using differential pulse voltammetry (DPV) technique on the surface of β‐Cyclodextrin modified glassy carbon (β‐CD/GC) electrode. β‐CD/GC electrode was prepared through an electrodeposition procedure and characterized by Fourier‐transform infrared spectroscopy (FT‐IR), Cyclic Voltammetry (CV), Field Emission Scanning Electron Microscopy (FESEM) and Energy‐dispersive X‐ray spectroscopy (EDS) techniques. Under optimum conditions, the β‐CD/GC electrode showed a good linearity as a function of the eflornithine concentration over the range from 5 to 100 μM with detection limit and quantification limit of 1.94 and 5.8 μM, respectively. Finally, the proposed protocol was confirmed to be successful in determination of eflornithine in human urine samples with good recovery, ranging from 97.2 % to 104.8 %.

The electrochemical oxidation of 4-tert-butylcatechol in the presence of a series of benzenamines has been evaluated in aqueous solution by means of cyclic voltammetry and controlled-potential coulometry. Results indicate that 4-tert-butylcatechol undergoes a two-electron oxidation at the surface of electrode to 4-tert-butylquinone followed by Michael addition reaction with benzenamine. This study has established the formation of aminoquinones as final products via the ECE mechanism. This procedure would be a good candidate for synthesis of aminoquinones in one-pot process, green conditions and good purity.

The electrochemical oxidation of N-acetyl-p-aminophenol (acetaminophen) (AC) has been studied in the presence of garlic and onion extracts using cyclic voltammetry, chronopotentiometry and chronocoulometry methods. The results revealed that the electrochemically generated toxic N-acetyl-p-benzoquinoneimine participates in the Michael addition reaction with organosulfur compounds contained in garlic or onion extracts and is converted to its reduced none toxic form(s). Based on these results, garlic and onion extracts can be useful for the treatment of acetaminophen poisoning before starting clinical treatments. O N O CH 3 OH HN O CH 3 CYP450 Cellular Toxicity Harmless Mercapturide OH HN O CH 3 S Cell Protein OH HN O CH 3 S 1 1a -2e --2H + Or Organosulfur Compound