Objective: Develop and validate a novel, sensitive, precise and straightforward UV spectrophotometric method to determine the amount of dioscin in tribulmon tablets. Methods: tribulmon tablets were prepared in the laboratory from medicinal plants Tribulus Terrestris L, native to Mongolia. Extract solutions were prepared from a single batch of tribulmon tablets, and the amount of dioscin was determined by UV spectrophotometric method using a calibration curve of known solutions of the dioscin. A chromogenic reaction was used to determine the amount of dioscin in the tablets. The maximum absorption was 201 nm. Validation parameters were evaluated following ICH guidelines. Results: The correlation coefficient of linearity for the UV spectrophotometric method was 0.9996. The standard working solution was linear from 3.2 to 18.9 μg/mL. The Limit of Detection (LOD) and Limit of Quantitation (LOQ) were 1.9 μg/ mL and 5.9 μg/mL, respectively. The recovery test accuracy was ranged from 97.9 to 100%. The %RSD for repeatability and intra-day precision was 0.45%, 0.13-0.50%, respectively. The %RSD for robustness was 0.26% to 0.40%, respectively. Conclusion: Based on the results of the proposed UV spectrophotometric method, the method we developed to determine dioscin content in tribulmon tablets from Tribulus Terrestris L is sensitive, precise, stable and straightforward. The test's validation parameters comply with ICH requirements.

The first official training of pharmacy technicians started in 1926 in Mongolia and pharmacy technicians were the only national pharmaceutical staff till the first national pharmacist graduated in 1954. Pharmacy technicians have played a valuable role in pharmaceutical care and service in Mongolia for the last century, which is still maintained nowadays. As of June 2022, one public university and one private medical institute implemented a pharmacy technician curriculum in Mongolia, and 512 pharmacy technicians had graduated in 2016-2020. Graduated pharmacy technicians are eligible to work in a community pharmacy, hospital, or pharmaceutical industry after being registered. They can also further their studies to get a B. Pharm. degree with an additional three years of training. As of 2020, a total of 2009 pharmacy technicians worked in Mongolia, 807 of them were employed in Ulaanbaatar and 1556 pharmacy technicians worked in the community pharmacies.