Overall aims of this research was to study the antibacterial activity of Voacanga foetida and then studied the distribution of the alkaloidal lombine produced in the plant, in which the samples were collected from three different regions. The antibacterial activities were assayed using agar well diffusion method. The distribution of the alkaloids in Voacanga foetida, especially lombine was studied on the basis of GC-MS in several temperature programs. The result showed that the leaves and the barks of Voacanga foetida were possessing antibacterial properties. The second result, GC-MS showed that alkaloidal lombine was produced stably in the barks while not in the leaves. The abundance of lombine in the base fraction of the barks was 6.06% (West Lombok), 74.91% (East Lombok), and 2.77% (North Lombok). The outcome will be then used as a platform for further studies investigating total uses of the plant especially in finding new materials for medicine.

Momordica charantia L seed is classified as the domestic waste even though it has many health benefits, namely as anti-tumor, anti-virus, and as a medicine to increase the body resistance. Therefore, this study was designed to examine the antioxidant activity of the ethanol extract of the seeds using the DPPH method. Furthermore, a GC-MS analysis will be carried out to determine the chemical compounds that play a role in the bioactivity. Based on the absorbance of DPPH in three repetitions, the average value of DPPH inhibition by 95% ethanol extract of bitter melon seeds was 60.45%, in which classified as a good radical absorbance. The GC-MS results showed that the ethanol extract of bitter melon seeds contained ten chemical compounds consisting of sesquiterpenes, fatty acids, and alkanes, which affecting the radical absorbance of M. charantia extract.

Abstrak: Upaya swamedikasi menjadi solusi dalam masa pandemi Covid-19 dalam memberikan daya imunitas tubuh. Didukung dengan sumber daya pekarangan rumah untuk dijadikan apotek hidup. Terlebih upaya swamedikasi dapat mengobati penyakit ringan, mencegah penyakit, merawat tubuh, dan menjaga kesehatan. Tujuan dilakukan sosialisasi adalah untuk memberikan pemahaman terkait upaya swamedikasi Covid-19 berbasis ramuan tradisional. Pelaksanaan kegiatan ini melibatkan mitra desa sejumlah 28 orang dari unsur Kelompok Taruna Tani dan Karang Taruna Desa Setungkep Lingsar. Metode yang dilakukan dengan diskusi dua arah serta memberikan paparan materi terkait covid19 dan pemanfaatan tanaman obat. Dalam kegiatan sosialisasi didapatkan ada peningkatan pemahaman 82% dari hasil pre-test menjadi 98% pada post-test, sehingga dapat disimpulkan terdapat peningkatan pemahaman sesudah diberikan edukasi terkait Covid-19 dan tanaman obat.Abstract: Self-medication efforts are a solution during the Covid-19 pandemic in providing body immunity. Supported by the resources of the yard of the house to be used as a living pharmacy. Moreover, self-medication efforts can treat minor illnesses, prevent disease, take care of the body, and maintain health. The purpose of the socialization is to provide an understanding of the efforts to self-medicate Covid-19 based on traditional ingredients. The implementation of this activity involved 28 village partners from elements of the Tani Group and the Setungkep Lingsar Village Youth Organization. The method is carried out with two-way discussions and provides exposure to material related to COVID-19 and the use of medicinal plants. In the socialization activity, it was found that there was an increase in understanding of 82% from the pre-test results to 98% in the post-test, so it can be concluded that there was an increase in understanding after being given education related to Covid-19 and medicinal plants.

This study is part of a research collaboration between Kyoto University and The University of Mataram, aiming to identify and to utilize the agarwood plants, i.e., Gyrinops versteegii from Indonesia. The study, specifically, aims to discover anticancer agents from the bark of G. versteegii from Lombok Island of Indonesia. There were three provenances of G. versteegii observed, namely Soyun, Pantai, and Buaya. Based on the Brine Shrimp Lethality Test (BSLT), G. versteegii Buaya showed the highest cytotoxicity with LC50 45,94 µg/ml. Meanwhile, G. versteegii Soyun and Pantai have LC50 75.86 µg/mL and 56.36 µg/mL respectively. A phytochemical study showed that the methanol extract of G. versteegii Buaya containing compounds from the group of alkaloid, flavonoid, steroid, triterpenoid, and saponin. The methanol extracts fractionated using Vacuum Liquid Chromatography into 10 fractions (F1-F10) and then retested BSLT. Among the fractions, F2 showed the best potency as an anticancer with LC50 64,12 µg/mL. Based on the GC-MS analysis, the cytotoxicity from both methanol extract and F2 is predicted to be influenced by the same compounds, namely, 1,4-Benzenediol,2-methyl, Pyridoxylamine, 2,3-Dimethylhydroquionone, Tetramethyl-p-benzoquinone, and Benzofuran. Overall, the bark of G. versteegii from Lombok Island has great potency as an anticancer.

ABSTRAK. Penelitian ini bertujuan untuk menganalisis kandungan senyawa kimia khususnya kandungan metabolit sekunder dari ekstrak metanol akar, batang, dan daun Nicotiana tabacum asal pulau lombok. Analisis dilakukan dengan menggunakan Gas Chrommatography-Mass Spectrophotometry (GCMS). Hasil analisis menunjukkan ada beberapa senyawa metabolit sekunder dari golongan alkaloid dan terpenoid. Nikotin atau 3-(1-metil-2-pirolidinil) piridin merupakan salah satu senyawa mayor yang berhasil diidentifikasi dengan % area masing-masing untuk akar, batang, dan daun berturut turut 18,35 %, 20,90 %, dan 40,59 %. Selain nikotin ada lima jenis senyawa alkaloid dari turunan asam nikotinat yang berhasil diidentifikasi, diantaranya; nikotrin, miosmin, anabasin, anatalin, dan 2,3'-bipiridin. Kelima senyawa alkaloid ini memiliki konsentrasi yang kecil. Selanjutnya pada ekstrak metanol akar dan daun Nicotiana tabacum juga berhasil diidentifikasi senyawa dari golongan terpenoid. Senyawa terpenoid tersebut yaitu leden dengan kerangka seskuiterpen dan b-4,8,13-duvatriene-1,3-diol dengan kerangka diterpenoid siklik. Nilai % area masing-masing adalah 0,46% dan 7,19 %. _____________________ ABSTRACT.This study aims to analyze the content of chemical compounds, especially the content of secondary metabolites from methanol extract of roots, stems, and leaves of Nicotiana tabacum from Lombok island. The analysis was carried out using Gas Chromatography-Mass Spectrophotometry (GCMS). The results of the analysis showed several compounds from the alkaloid group, fatty acids and its derivatives, terpenoids, and alcohol. Nicotine or known as 3-(1-methyl-2-pyrrolidinyl)-pyridine is one of the major compounds that was successfully identified with % area from roots, bark and leaves respectively 18.35%, 20.90%, and 40, 59%. Besides nicotine, there are five types of alkaloid compounds which are derivatives of nicotinic acid that have been successfully identified. Furthermore, In the methanol extract of Nicotiana tabacum roots and leaves, compounds from the terpenoid group have also been identified. There are leden from sesquiterpen and b-4,8,13-duvatriene-1,3-diol from the cyclic diterpenoid. The % area value is 0.46% and 7.19%, respectively. _____________________ PENDAHULUANTembakau dengan spesies Nicotiana tabacum, merupakan jenis tanaman semak yang tumbuh di daerah dengan iklim tropis dan subtropis. Nicotiana tabacum secara komersil dibududayakan untuk memperoleh daun tembakau yang merupakan bahan dasar pembuatan rokok. Selain itu, secara etnobotani tanaman ini juga dimanfaatkan sebagai insektisida, obat penghilang rasa nyeri, obat penenang, obat sakit gigi, dermatitis, dan untuk pengobatan gangguan pencernaan (Rodgman and Perfetti, 2008;Groark, 2010).