Methicillin resistant Staphylococcus aureus (MRSA) adalah jenis bakteri yang timbul karena resistensi terhadap antibiotik, di pusat layanan kesehatan penyebaran bakteri-bakteri yang mengalami resistensi tersebut dapat melalui sentuhan pasien dengan tenaga medis atau peralatan medis yang digunakan di fasilitas layanan kesehatan. Penelitian ini bertujuan untuk mendeteksi keberadaan MRSA, resistensinya terhadap antibiotik serta mendeteksi keberadaan MRSA SSCmec tipe III. Sampel dari diafragma stetoskop dan manset tensimeter di ruang rawat inap RSUD Provinsi NTB, diperiksa secara mikrobiologi dan secara molekuler menggunakan teknik PCR (Polimerase Chain Reaction) menggunakan primer SSCmec Tipe III. Selama bulan Januari hingga Februari 2018 didapatkan sebanyak 20 sampel swab diafragma stetoskop dan 18 swab manset tensimeter. Pengambilan sampel dilakukan pada akhir Januari hingga pertengahan Februari 2018.  Hasil penelitian ditemukan sebanyak 2 isolat dari swab diafragma stetoskop (10%) dan 2 dari manset tensimeter (11.1%)  terdeteksi sebagai MRSA. Dari empat isolate tersebut semuanya masih sensitive terhadap Vancomycin, tiga isolate  resisten Clindamycin, Cefoxitin, dan Oxacilin, serta 1 isolat resisten Cefoxitin dan Oxacilin. Hasil pemeriksaan PCR pada 4 isolat MRSA menunjukkan tidak ditemukan MRSA SSCmec tipe III.

: the utilization of antibacterial active substances from several plants is increasingly not only as the ingredients of medicine, but its utilization is also utilized for an antibacterial ingredient for preventive action, one of them is hand sanitizer material. This research focused to find the antibacterial active substances alternative from Muntingia calabura leaves extract. The data were analyzed descriptively including the inhibitory of ethanol absolute extract and ethanol 95% of M. Calabura against isolate clinical bacteria by Kirby Bauer method and the type of coumpund that contains in M.calabura leaf by thin layer chromatography utilizingeluen n-hexan- Methanol. Etanol absolute extract M.calabura leaf showing the average inhibition zone against Pseudomonas aeruginosa 15.67 mm, Staphylococcus aureus 19.33 mm and Escherichia coli 13 mm. While, The etanol extract 95% showing higher inhibition Pseudomonas aeruginosa 19.67 mm, Staphylococcus aureus 19.33 mm and Escherichia coli 16.67 mm. This inhibitory zone was slightly lower than chlorhexidine gluconate with an average of 20-24 mm against the three bacteria that utilized in the test but belongs to the strongly sensitive category for natural materials according to Mukherjee (1988). From thin layer chromatography profile with eluen n-hexan: methanol found three compounds that were in the range of Rf value 0.4; 0.5 and 0.7. The Conclusion for this study is the bioactive material from etanol 95% extract M.calabura leaf can be optimized to the hand sanitizer active compound candidate.