Objective: The objective of the study was to understand biomolecular interactions of Bromelain and its networking with p53 and β-catenin by a computational method of analysis in Hepatocellular carcinoma (HCC) condition. Methodology: The protein interaction partners for p53 and β-catenin involved in the progression of HCC were collected from National Center for Biotechnology Information. We collected data points and standardized the data points for our data analysis from the public database. We used Cytoscape 3.8.2 version plug-in for constructing a Protein-Protein interaction network. We constructed a pathway network using Biorender.com. Results: The protein interactions concerning p53 and β-catenin are identified and a network is constructed. A total of 18 and 34 nodes were identified which are involved in down-regulation and up-regulation of β-catenin and a total of 30 and 27 nodes for homosapiens are identified which are involved in the downregulation and upregulation of the p53 gene. We identified different pathways which trigger and impact the p53 and Wnt/β- catenin signaling pathways as potential target sites for Bromelain to arrest the progression of cancer Conclusion: In conclusion, our in silico studies anti-cancer activity of Bromelain in HCC relating its effect on apoptosis, cell differentiation, mesenchymal transition, p53 signaling, and Wnt/β-catenin signaling pathways.

Malachite Green (MG), a cationic synthetic dye is considered hazardous when discharged into the water bodies without any adequate treatment. It can affect the multiple segments of the environment leading to irreversible persistent changes. So, there is a need for remediation with cost-effective method to remove dyes from effluents. Adsorption is one such technique to remove dyes from wastewater and is effective and economical. The present study describes the removal of MG cationic dye from wastewater using eco-friendly and biodegradable lignin extracted from hydrothermally treated rice straw by adsorption process. Functional group analysis and morphological characterisation was done to the extracted lignin after quantification. The maximum percent removal of MG 92 ± 0.2 % was observed from a series of batch experiments at optimum process parameters of: contact time 80 min, initial dye concentration 50 ppm, lignin dosage 0.25g, pH 7, temperature 300c and with 100 rpm agitation speed. The adsorption kinetics and isotherms were determined for the experimental data using four kinetic models (pseudo-first-order, second order, pseudo-second-order and intra-particle diffusion model) and two isotherm models (Langmuir and Freundlich). The results suggested that the kinetics data fit to the pseudo-second-order kinetic model with the maximum adsorption capacity 36.7 mg/g and the two isotherm models were applicable for the adsorption of MG onto the lignin. Additionally, the thermodynamic parameters ΔSo, ΔHo and ΔGo were evaluated. Therefore, lignin which is an environmental friendly and low cost carbon material that can be used as an adsorbent for dye removal.