Chlorella vulgaris is known as one of the fastest growing microalgae, and is used in this study for the removal of residual ammonia (NH 3 )/ ammonium ion (NH 4 + ) present in the wastewater effluent collected from a local wastewater treatment plant, Mill Creek plant in Cincinnati, OH. The wastewater effluent contained 8.05 ± 0.16 mg/L of nitrogen concentration in the form of ammonia/ ammonium ion, 50.18 ± 0.82 mg/L of total inorganic carbon concentration, and 1.84 ± 0.10 mg/L of orthophosphate concentration at pH 7.34 ± 0.05. This study demonstrates that Chlorella vulgaris has the capability to diminish nitrogen concentration in a form of ammonia/ ammonium ion during its growth while also consuming total inorganic carbon concentration. The Monod equation is used to describe its kinetics for growth and stationary phases using nitrogen as a limiting substrate.
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