Unsymmetrical Schiff baseH 2 L 1 =2-((8-((E)-1-((2-hydroxy phenyl) ethylidene amino) naphthalen-1ylimino) methyl)-6-methoxyphenol and H 2 L 2 =1-((E)-(8-((E)-1-(2-hydroxy phenol) ethylidene amino) naphthalen-1-ylimino)methyl)naphthalen-2-ol which derived from 1,8-diaminonaphthalene and 2-hydroxy acetophenone and then with 2-hydroxy-3-methoxy benzaldehyde or 2-hydroxy-1naphthaldehyde and their complexes of the type [M L n ], where n=1,2 and M=Co(II) Ni(II),Cu(II)and Zn(II) have been synthesized.Adduct complexes of the type [ML n (py) 2 ] were also prepared in(1:2)(complex: py) molar ratio. The complexes and adducts were characterized by elemental analysis (C.H.N), metal content, (IR,UV-Vis, 1 H-NMR)spectroscopy, conductivity and magnetic measurements. The resulting data suggested that the Schiff bases containing ONNO donor atoms act as dibasic tetradentate ligands through coordination with metal ions. Conductivity data in DMF solution showed that all complexes are non-electrolyte. Magnetic moment and electronic spectra dada indicate that the complexes have either squar planer or octahedral geometry, the ligands and their complexes gram negative bacteria (Salmonella typhi andEscherichia coli).