The novel coronavirus (SARS-CoV-2/ COVID-19) rapidly infects people across the world, leading to a massive public reaction. Peoples’ knowledge, attitudes, and practices (KAP) towards measures for prevention of the spread of COVID-19 are the most important for the control and prevention of the pandemic infectious disease. This study aimed to assess the knowledge, attitude & practices (KAP) towards measures for prevention of the spread of COVID-19 among Bangladeshi residents during the COVID-19 pandemic. An online survey of 1765 respondents was conducted through the social media networks of authors. The respondents were older than 18 years, with an average age of 24.88 years (SD: 6.30). Approximately 15% of our participants received online training. The mean knowledge score was 14.49 (SD: 1.8, ranging: 0-17), and the overall correct response rate on this knowledge test was 85%. About 67.2 % scored well (above the mean: 4.5, range: 1-5) regarding the practices. To avoid the infection, 96.6% wear mask outside the home, and 98.7% washed hand with soap when came back. COVID-19 knowledge score was significantly associated with a lower risk of (OR: 0.87, 95% CI: 0.79-0.92) of negative attitudes. Again, the awareness score was associated with a lower likelihood of (OR: 0.94, 95% CI: 0.87-0.98) of poor practices. Online health education programs focusing on young people, housewife and people with less education may potentially improve the attitudes and practices to control the COVID-19 pandemic in long term in such a low resource setting.

Mucosal-associated invariant T (MAIT) cells are unconventional T lymphocytes with a semi-conserved TCR-alpha, activated by the presentation of vitamin B metabolites by the MHC-I related protein, MR1, and with diverse innate and adaptive effector functions. The role of MAIT cells in acute intestinal infections, especially at the mucosal level, is not well known. Here, we analyzed the presence and phenotype of MAIT cells in duodenal biopsies and paired peripheral blood samples, in patients during and after culture-confirmed Vibrio cholerae O1 infection. Immunohistochemical staining of duodenal biopsies from cholera patients identified MAIT cells in the lamina propria, but not in the lining of villous and crypt epithelia. We observed significantly higher frequencies of duodenal MAIT cells at the acute stage (day 2) of V. cholerae infection as compared to late convalescence (day 30, p = 0.0049). By flow cytometry, we showed that duodenal MAIT cells are more activated than peripheral MAIT cells (p < 0.01 across time points), although there were no significant differences between duodenal MAITs at day 2 and day 30. We found fecal markers of intestinal permeability and inflammation to be correlated with the loss of duodenal (but not peripheral) MAIT cells, and single-cell sequencing revealed differing T cell receptor usage between the duodenal and peripheral blood MAIT cells. In summary, we show that MAIT cells are present and highly activated in the lamina propria of the duodenum during V. cholerae infection. Future work into the trafficking and tissue-resident function of MAIT cells is warranted.

IntroductionThe rapid spread of SARS-CoV-2 coupled with inefficient testing capacities in Bangladesh has resulted in a number of deaths from COVID-19-like symptoms that have no official test results. Insufficient test sites and healthcare facilities catered to COVID-19 has led to feelings of fear and frustration in those who are sick. This study was the first study which explored the mental health of adults with the most common COVID-19-like symptoms in Bangladesh.MethodsThis retrospective case control study gathered data via an online survey to explore the mental health of Bangladeshi adults with symptoms akin to COVID-19. Level of stress, anxiety symptoms, and depressive symptoms were measured with the DASS-21. Chi-square tests and multivariate logistic regression was performed to examine the association of variables. ResultsThe prevalence rates of anxiety symptoms, and depressive symptoms of overall population were 26.9% and 52.0% respectively and 55.6% reported mild to extremely severe levels of stress. Multivariate logistic regression determined that respondents with COVID-19-like symptoms (case) reported higher odds for stress level (AOR: 2.043; CI: 1.51-2.76), anxiety symptoms (AOR: 2.770; CI: 2.04-3.77) and depressive symptoms (AOR: 1.482; CI: 1.12-1.96) than asymptomatic respondents (control). ConclusionPatients with symptoms like those of COVID-19 should be prioritized in the healthcare setting in order to reduce mental health difficulties throughout the pandemic.