Flash flooding (FF) along with bacterial blight (BB) outbreak are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem. The presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL. In this context, the marker-assisted improvement of plant resistance/tolerance has been proven as one of the most promising strategies towards the development of sustainable cultivars. The present study demonstrates the marker-assisted introgression of the submergence tolerant (Sub1) and three bacterial blight resistant genes (Xa21 + xa13 + xa5) into the genetic background of Hasanta, a long duration popular rice variety in the eastern coastal region of India. The rice genotypes, Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21 + xa13 + Xa7 + xa5 + Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta; recurrent parent (RP) was used as donor. The forward analysis of target genes in F1s, IC1F1s and backcross (BC) generations was performed by linked/genic markers (Sub1bc2; pTA248, xa13prom and RM122), whereas background recovery of RP in each BC and segregating generations was performed utilizing 108 hypervariable SSR markers. Intervened speed breeding (SB) strategy and intensive phenotyping could lead the development of near isogenic lines (NILs) as to the RP in all basic traits. The performance of the near isogenic lines (NILs, BC2F3 and BC2F4), HS 232-411-391-756-37, HS 232-411-391-809-8, HS 232-411-391-756-18, HS 110-224-197-10-36, HS 232-411-391-809-81, HS 110-224-197-10-41 and HS 232-411-391-809-63 establishes the utility of marker-assisted backcross-breeding (MAB) and SB in accelerated trait introgression. The introgressed lines carrying Sub1 + Xa21 + xa13 + xa5 showed 76% to 91% survival under 14 days of submergence and durable BB resistance (percent disease index-PDI of 2.68 ± 0.26 to 6.22 ± 1.08 and lesion length, LL of 1.29 ± 0.12 to 4.2 ± 0.64 cm). Physiological analysis revealed that improved NILs, carrying Sub1 gene conquered adaptive physiological modulations, had reduced the consumption of soluble sugar and the degradation of total chlorophyll contents (TCC), and an enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes. The pyramided lines attained complete product profile of RP, that will contribute to sustainable rice production under RSL, particularly in the coastal region that has substantial acreage under the variety Hasanta.
A field experiment was conducted at the instructional farm of Krishi Vigyan Kendra Jajpur, Odisha during 2016 to study the effect of integrated nutrient management on disease and pest incidence and yield of okra (Abelmoschus esculentus (L.) Moench) variety Pusa A-4. From the experiment it was observed that treatment T7 [ RDF(75%)+ (25%) neem oil cake] has significant effect on disease incidence and pest population by reducing incidence of YVMV from 32.42% to 19.56%, jassid population from 11.57 to 6.53 per three leaves,mite population from 11.98 to 8.11 per three leaves, white fly population from 13.61 to 6.64 per three leaves and fruit borer from 17.94 % to 7.94 %. Maximum number of fruits per plant 15.02 was recorded in T7. Fruit yield per hectare varied significantly and was maximum with T7 (10.49 ton) and was minimum with T11 (7.30 ton). T7 (10.49 ton) and T5 (9.85 ton) and T10 (9.65 ton) were at par with each other. Combined use of 75 % RDF as inorganic fertilizer with 25 % RDF through neem oil cake (T7) recorded highest gross income of Rs.1,45,770/-, net income Rs.85,458 /-per hectare and maximum benefit cost ratio 2.42.
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