A novel nanoparticle-based electrochemical immunoassay of carbohydrate antigen 125 (CA125) as a model was designed to couple with a microfluidic strategy using anti-CA125-functionalized magnetic beads as immunosensing probes. To construct the immunoassay, thionine-horseradish peroxidase conjugation (TH-HRP) was initially doped into nanosilica particles using the reverse micelle method, and then HRP-labeled anti-CA125 antibodies (HRP-anti-CA125) were bound onto the surface of the synthesized nanoparticles, which were used as recognition elements. Different from conventional nanoparticle-based electrochemical immunoassays, the recognition elements of the immunoassay simultaneously contained electron mediator and enzyme labels and simplified the electrochemical measurement process. The sandwich-type immunoassay format was used for the online formation of the immunocomplex in an incubation cell and captured in the detection cell with an external magnet. The electrochemical signals derived from the carried HRP toward the reduction of H(2)O(2) using the doped thionine as electron mediator. Under optimal conditions, the electrochemical immunoassay exhibited a wide working range from 0.1 to 450 U/mL with a detection limit of 0.1 U/mL CA125. The precision, reproducibility, and stability of the immunoassay were acceptable. The assay was evaluated for clinical serum samples, receiving in excellent accordance with results obtained from the standard enzyme-linked immunosorbent assay (ELISA) method. Concluding, the nanoparticle-based assay format provides a promising approach in clinical application and thus represents a versatile detection method.

The demand for CO 2 /C 2 H 2 separation, especially the removal of CO 2 impurity, continues to grow because of the high-purity C 2 H 2 required for various industrial applications. The adsorption separation of C 2 H 2 and CO 2 via porous materials is gaining a considerable attention as it is more energy-efficient compared with cryogenic distillation. The ideal porous materials are those that preferentially adsorb CO 2 over C 2 H 2 ; however, very few adsorbents meet such requirement. Herein, two isostructural cyclodextrin-based CD-MOFs (CD-MOF-1 and CD-MOF-2) were demonstrated to have an inverse ability to selectively capture CO 2 from C 2 H 2 by single-component adsorption isotherms and dynamic breakthrough experiments. These two MOFs showed excellent adsorption capacity and benchmark selectivity (118.7) for CO 2 /C 2 H 2 mixture at room temperature, enabling the pure C 2 H 2 to be obtained in only one step. This work revealed that these materials were promising adsorbents for obtaining high-purity C 2 H 2 via selectively capturing CO 2 from C 2 H 2 .

Introduction of Ag(i) ions into a sulfonic acid functionalized MOF ((Cr)-MIL-101-SO3H) significantly enhances its interactions with olefin double bonds, leading to its much higher selectivities for the separation of C2H4-C2H6 and C3H6-C3H8 at room temperature over the original (Cr)-MIL-101-SO3H and other adsorbents at room temperature.