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Neuroblastoma is a common malignancy and frequently affects children, leading to a low survival rate. Long noncoding RNAs (lncRNAs) are reported to be closely related to cancer progression. The purpose of this study was to explore a novel mechanism of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in neuroblastoma. NEAT1 was upregulated in neuroblastoma cell lines (IMR32 and SK-N-SH). Overexpression of NEAT1 increased proliferation inhibited by cisplatin and decreased apoptosis promoted by cisplatin. MicroRNA-326 (miR-326) was a target of NEAT1 and miR-326 reintroduction abolished the effects of NEAT1 overexpression on cell proliferation and apoptosis. Moreover, NEAT1 overexpression activated Janus kinase/signal transducer and activator of transcription 3 (JAK1/STAT3) signaling pathway through absorbing miR-326. Besides, NEAT1 overexpression promoted tumor growth in vivo through stimulating the expression of p-JAK1 and p-STAT3 but inhibiting miR-326 expression. NEAT1 accelerated proliferation and weakened apoptosis of neuroblastoma cells treated by cisplatin by targeting miR-326 through activating JAK1/STAT3 signaling pathway, suggesting that NEAT1 was a potential biomarker against neuroblastoma.

In this study, four Theaceae tree species were processed by setting up two hydrogen fluoride concentration gradient (including controls), the actual quantum yield (Y (II)) of PSII, the relative electron transport rate (ETR), non-regulatory energy dissipation quantum yield (Y(NO)), regulatory energy dissipation quantum yield (Y(NPQ)), non-photochemical quenching coefficient (NPQ) and the relative electrical conductivity and other physiological characteristic value were measured, and fuzzy membership function method was applied for comprehensive evaluation and stress resistance sequencing of these four species, which could help to explore the impact of HFstress on physiological indicators of Theacea tree seedlings, and effectively screen out tress species with high resistance to hydrogen fluoride; besides, it could provide the basis for selection of planting trees with the purpose of environmental protection, and also provide a reference for HF stress mechanisms study. This study showed that, under the circumstance of 500 ppm hydrogen fluoride stress, chlorophyll fluorescence indicator of Theaceae tree species presented a downward trend on the whole, which concretely embodied in varying degrees of decline of Y (II) of PSII, ETR, Y (NO), Y (NPQ), NPQ. And the cell membranes of trees species were damaged and the membrane permeability increased. Except for Schima superba, the relative conductivity value of other species showed an upward trend. The capacity of these four species in anti-hydrogen fluoride contamination was Tutcheria championi first, Camellia oleifera second, Schima superba third, Gordonia axillaries is the last.